Sequences from confirmation amplicon


Confirmation sequences for line 080E07 and hit At2g46210 are shown.
Line ID 080E07
Locus At2g46210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18978474
Primers used for PCR AU26 - 8474, show primer details
   
Sequence Name 08-K106284-0022-080-E07C-8409
GenBank Accession LN489740 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106284-0022-080-E07C-8409
CGGACTGAGCCTTTCAATTGAATATTCCTGAAAACGGAAGGAACGAGACTAAGAGAGGGA
ACCATGTCCAGAAAACTAAAATTCCGGCGATGTTCAAGGCCCGATCTGGGACGTGGCGTT
TCGAGAATAGCAATAGGAATGTTTGGATGAAGAGGTTGATTCTTCCGACGCACATTACTG
GGTAAAATGTCCAGTGTTGGTAGCTGATTAAGAATCGAGCTAGAGGATCGAATGTTAATT
TCCTGCCATAGAAACGTGACGTCATCGAATTGAAGAATTTTGTAGAGACGGCGAAGATCG
GGATGTGTTGTAGATCCGGATCGTGGTCAAGGCTGTTACAAGCGATGTGGTGAGCGTTGT
GCGTCCATTTCCACCACGCGATTGAGATGCCGGTGAGACAGTTACCGGAGAGAAGCTGGA
TTAGTTTGTNACACGGT
   
Sequence Name 08-K106285-0022-080-E07C-AU26
GenBank Accession LN489741 [GenBank]
Sequencing primer gene specific primer, AU26: 5'-AACCGTGTAACAAACTAATCCAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106285-0022-080-E07C-AU26
GGCATTCATCGCGTGGTGGAATGGACGCACAACGCTCACCACATCGCTTGTCACTGCCTT
GACCACGATCCGGATCTACAACACATCCCGATCTTCGCCGTCTCTACAAAATTCTTCAAT
TCGATGACGTCACGTTTCTATGGCAGGAAATTAACATTCGATCCTCTAGCTCGATTCTTA
ATCAGCTACCAACACTGGACATTTTACCCAGTAATGTGCGTCGGAAGAATCAACCTCTTC
ATCCAAACATTCCTATTGCTATTCTCGAAACGCCACGTCCCAGATCGGGCCTTGAACATC
GCCGGAATTTTAGTTTTCTGGACATGGTTCCCTCTCTTAGTCTCGTTCCTTCCGTTTTCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCACGNTTATTA
Primers used for PCR 10ax - 3144, show primer details
   
Sequence Name 08-K106867-0022-080-E07C-3144
GenBank Accession LN489751 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K106867-0022-080-E07C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACA
TCTTCGTCTTCGTGAGCTTCGCCGTCACGGCGATTCAACATGTTCAGTTCTGTTTAAACC
ATTTCGCAGCAGATGTGTATACCGGTCCACCAAACGGAAACGATTGGTTTGAGAAACAAA
CCGCCGGTACGCTTGATATATCGTGTAGATCGTTTATGGATTGGTTCTTTGGTGGATTGC
AGTTTCAGTTAGAGCATCATTTGTTTCCTCGGCTTCCTCGTTGTCATCTCCGGACAGTAT
CGCCGGTGGTTAAGGAGCTTTGTAAGAAGCATAATCTACCGTATAGGAGTCTTTCGTGGT
GGGAAGCTAATGTGTGGACGATTAGGACTTTGAAGAACGCGGCGATTCAAGCTAGAGATG
CGACTAATCCTGTGTTGAAGAACTTGCTTTGGGAAGCTGTGAACACGCATGGCTAAAAAG
GTGTTTCGTTTGCACAAGTAC
   
Sequence Name 18-K106868-0022-080-E07C-10ax
GenBank Accession LN494325 [GenBank]
Sequencing primer gene specific primer, 10ax: 5'-AGTACTCGACAAACGAAACACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K106868-0022-080-E07C-10ax
GCTTCCAAGCAGTTCTTCAACACAGGATTAGTCGCATCTCTAGCTTGAATCGCCGCGTTC
TTCAAGTCCTAATCGTCCACACATTAGCTTCCCACCACGAAAGACTCCTATACGGTAGAT
TATGCTTCTTACAAAGCTCCTTAACCACCGGCGATACTGTCCGGAGATGACAACGAGGAA
GCCGAGGAAACAAATGATGCTCTAACTGAAACTGCAATCCACCAAAGAACCAATCCATAA
ACGATCTACACGATATATCAAGCGTACCGGCGGTTTGTTTCTCAAACCAATCGTTTCCGT
TTGGTGGACCGGTATACACATCTGCTGCGAAATGGTTTAAACAGAACTGAACATGTTGAA
TCGCCGTGACGGCGAAGCTCACGAAGACGAAGATGTCAAACACTGATAGTTTAAACCGAA
GGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCC
TTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGT
GGAGATATC

Last Updated on 06.12.2018 16:11