Sequences from confirmation amplicon


Confirmation sequences for line 080F08 and hit At1g53282 are shown.
Line ID 080F08
Locus At1g53282 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19868143
Primers used for PCR 01oh - 8474, show primer details
   
Sequence Name 27-K114782-0022-080-F08C-01oh
GenBank Accession LN498512 [GenBank]
Sequencing primer gene specific primer, 01oh: 5'-TTCCGAGTGGAGTAGATGAGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114782-0022-080-F08C-01oh
TTTGCATTGATGAGATGTTGCTTTGTATTGATGAACATTTTGAGTCCGATAATTTATAAT
ACTACAAAAGTAAACCAAAAATGTTGGTTTTCACTCTTCTATCTTCTTTTAATCTTTGCT
TCTTTTATAATTGGTATAAAAAAAAGTTTTATAATTTAAAAAATATTTTTATTGATATGA
AAACTTCATCAATCTTACAAATATCTCGACTATTTTGTCAACTTATCTACCCATTTATGT
TTCTCATTTCTATAGTCTCCATCCTGCAATTTTTTTATATAAATCATGTCAATTTCTTTA
TACAAAATATTTTTTTAATCAAACTAAATAAAACAAGTGAAGAGATTATTACTGGGATGA
TTGAGCTGACTGAAGTCTACGACATTTGCGGTCACATTTATCTTTACAGTATTTTTCTGG
ATGTACATTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCACAA
   
Sequence Name 27-K114769-0022-080-F08C-8409
GenBank Accession LN498511 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K114769-0022-080-F08C-8409
GCTCAATTCATCCCAGTAATAATCTCTTCACTTGTTTTATTTAGTTTGATTAAAAAAATA
TTTTGTATAAAGAAATTGACATGATTTATATAAAAAAATTGCAGGATGGAAAACTATAGA
AATGAGAAACATAAATGGGTAGATAAGTTGACAAAATAGTCGAGATATTTGTAAGATTGA
TGAAGTTTTCATATCAATAAAAATATTTTTTAAATTATAAAACTTTTTTTTATACCAATT
ATAAAAGAAGCAAAGATTAAAAGAAGATAGAAGAGTGAAAACCAACATTTTTGGTTTACT
TTTGTAGTATTATAAATTATCGGACTCAAAATGTTCATCAATACAAAGCAACATCTCATC
AATGCAAATCTGCACTTTGCATAATTTTCAACATGCAAATCTC

Last Updated on 06.12.2018 16:11