Sequences from confirmation amplicon


Confirmation sequences for line 081A08 and hit At5g05840 are shown.
Line ID 081A08
Locus At5g05840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1757768
Primers used for PCR 25jd - 8474, show primer details
   
Sequence Name 73-K113077-0022-081-A08C-25jd
GenBank Accession LN512931 [GenBank]
Sequencing primer gene specific primer, 25jd: 5'-AACCATCTATGGTCCACCACTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113077-0022-081-A08C-25jd
TTAGTTGGTTCCCAAATAGATATATTTGTTAACAAAGATATTGCTACTTGATTTTCTCCA
AATAGTATTTTTGTTATAACAAAGTAGACTTTTGTTCCCATTACCAAGTGTATAGTTATT
TACAACCTAATAAGATCTTCTTTTATTGTCACCAAACCAATAGCATTTGTTTGTTTTGGG
AAATTTGTAATAATGATGAATTAAGTTACTTGTCGTTGACTTGTTTCAAGTATCTTTGTG
TTTAGTACTTGTCTGAATGGAATCATTACAAAGGTTTCAGACCTTAATCGTTGAAACAAA
ATGAAACAGGGTCTTGATCCAAAATCAACGGCTAATCTATTTGCAAGATCAGTATGCATG
GGAGAGAAGAAGATAAATGATGAAGACTGTTTCATACTCAAACTTGATGCTGAGCCATCG
GCGCTTAAGGCGAGAAGCAGCAGCAACACATGCACACTGTGTCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 46-K113075-0022-081-A08C-8409
GenBank Accession LN505917 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K113075-0022-081-A08C-8409
TGCTGCTTCTCGCCTTAAGCGCCGATGGCTCAGCATCAAGTTTGAGTATGAAACAGTCTT
CATCATTTATCTTCTTCTCTCCCATGCATACTGATCTTGCAAATAGATTAGCCGTTGATT
TTGGATCAAGACCCTGTTTCATTTTGTTTCAACGATTAAGGTCTGAAACCTTTGTAATGA
TTCCATTCAGACAAGTACTAAACACAAAGATACTTGAAACAAGTCAACGACAAGTAACTT
AATTCATCATTATTACAAATTTCCCAAAACAAACAAATGCTATTGGTTTGGTGACAATAA
AAGAAGATCTTATTAGGTTGTAAATAACTATACACTTGGTAATGGGAACAAAAGTCTACT
TTGTTATAACAAAAATACTATTTGGAGAAAATCAAGTAGCAATATCTTTGTTAACAAATA
TATCTATTTGGGAACCAACTAATGTATACTAGTTATAAGTATATATTTTTGGTTGTTGTT
GTTAGTGGTGGACTAAGNATGGTT

Last Updated on 06.12.2018 16:11