Sequences from confirmation amplicon


Confirmation sequences for line 081A08 and hit At5g19620 are shown.
Line ID 081A08
Locus At5g19620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6625830
Primers used for PCR 09pg - 8474, show primer details
   
Sequence Name 25-K115435-0022-081-A08C-09pg
GenBank Accession LN497587 [GenBank]
Sequencing primer gene specific primer, 09pg: 5'-ATGGGAATCATGGAAGATGTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115435-0022-081-A08C-09pg
AGTAGTATGAGTGAAGAAAGTATTTTCCTTGCTTATCCTTAGGCACAGTAACAAAAGGTT
TTGACAGTGTCTGATTCTGAAACTTTCTTCTTTTCCTTGTTTTTTATTCCTGTTAGATAG
TGGGAAGGTTGATTTGATCATGAACTGTGTCGAGCGTCCCAGTGGAGGCTTCTCTGCTGG
TGGTGGGATATCTAGTGGGTAAGTTTTAGTTTGTTTGCTACTTACAGCGATTCAATTTTT
TTCTTTTGTAGCTTGATGACAACTTTTGTCCAATTCTTTTGTAGGATCACAAGTGGCCCC
CTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 09-K115434-0022-081-A08C-8409
GenBank Accession LN490320 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115434-0022-081-A08C-8409
ATAGGGGGCCACTTNTGATCCTACAACAGAATTGGACAAAAGTTGTCATCAAGCTACAAA
AGAAAAAAATTGAATCGCTGTAAGTAGCAAACAAACTAAAACTTACCCACTAGATATCCC
ACCACCAGCAGAGAAGCCTCCACTGGGACGCTCGACACAGTTCATGATCAAATCAACCTT
CCCACTATCTAACAGGAATAAAAAACAAGGAAAAGAAGAAAGTTTCAGAATCAGACACTG
TCAAAACCTTTTGTTACTGTGCCTAAGGATAAGCAAGGAAAATACTTTCTTCACTCATAC
TACTGCACCTTAAGCACAAGATGAAAATTAAAAGTATTAGCTGAAAAGAAAGAAATATAT
TTACACACCTCCTGCAGGTTGAGGAATAATACTAACATCTT

Last Updated on 06.12.2018 16:11