Sequences from confirmation amplicon


Confirmation sequences for line 081A09 and hit At4g37340 are shown.
Line ID 081A09
Locus At4g37340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17566067
Primers used for PCR AY30 - 8474, show primer details
   
Sequence Name 22-K020068-022-081-A09C-8409
GenBank Accession LN495920 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K020068-022-081-A09C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAATTGACCATC
ATATGTTAAATATTTGCTACAATTCACTGAAATTCACCTATAAGCAGAGATTGACTAGGG
ACTAAGGATTCTGATTATAATAAATTTTGCTAATTAGCATAAAATTGGAACAAAGAACAA
AGAACAAACTTACGAGTGAAGAGTTCCGTGAGAGACAAGCTATAAGCCGGTGGATCTCGT
CTCGGCGGATAGATGAAAAGCTATTGAGACGGTGAGCCGAGAATATCTCAACAGCGCCAA
TACGGCGGAGATTTCTCCAATGGTCGCCGTACGAAGCTGTAACAACCGTTGTGCAGCCAT
ATGAGATGTGTTTTGAAGCA
   
Sequence Name 21-K020068-022-081-A09C-AY30
GenBank Accession LN495473 [GenBank]
Sequencing primer gene specific primer, AY30: 5'-CCTTGCTTCAAAACACATCTCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K020068-022-081-A09C-AY30
GCTGCACACACGGTTGTTACAGCTTCGTACGGCGACCATTGGAGAAATCTCCGCCGTATT
GGCGCTGTTGAGATATTCTCGGCTCACCGTCTCAATAGCTTTTCATCTATCCGCCGAGAC
GAGATCCACCGGCTTATAGCTTGTCTCTCACGGAACTCTTCACTCGTAAGTTTGTTCTTT
GTTCTTTGTTCCAATTTTATGCTAATTAGCAAAATTTATTATAATCAGAATCCTTAGTCC
CTAGTCAATCTCTGCTTATAGGTGAATTTCAGTGAATTGTAGCAAATATTTAACATATGA
TGGTCAATTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGGAAGCATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR AY29 - 8474, show primer details
   
Sequence Name 20-K020068-022-081-A09C-oriL
GenBank Accession LN495022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K020068-022-081-A09C-oriL
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATACTCATTTTTAATATTTA
CATGTAGCATTATAAATTTTGCAGGAATTTACAAAGGTTGAGATGAAATCAATGTTTTCT
AACTTAACATTCAACAACATTATAAGAATGTTGGCTGGAAAATGTTACTACGGAGATGGT
GCAGAGGATGACCCGGAGGCTAAACGCGTGCGGGAGCTTATAGCAGAGGGAATGGGTTGT
TTTGGTGCTGGAAACACTGCTGATTATTTACCGATCCTGACTTGGATCACAGGTTCCGAG
AAACGGATAAAGAAGATCGCTAGTAGGCTTGATGAGTTCTTGCAGGGATTGGTCGATGAA
AGACGAGAAGGGAAGGAGAAAAGGCAGAACACAATGGTTGATCACTTGCTTTGTCTGCAA
GAAACCCAACCCGAGTACTACACGGACAACATTATCAAAGGAATCATGCTCGTGAGTAAC
ATTTTAAGTTCATTAGTCTTTGT
   
Sequence Name 19-K020068-022-081-A09C-AY29
GenBank Accession LN494568 [GenBank]
Sequencing primer gene specific primer, AY29: 5'-TCCCATACCAAATGTGACAATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K020068-022-081-A09C-AY29
CTAATGAACTTAAAATGTTACTCACGAGCATGATTCCTTTGATAATGTTGTCCGTGTAGT
ACTCGGGTTGGGTTTCTTGCAGACAAAGCAAGTGATCAACCATTGTGTTCTGCCTTTTCT
CCTTCCCTTCTCGTCTTTCATCGACCAATCCCTGCAAGAACTCATCAAGCCTACTAGCGA
TCTTCTTTATCCGTTTCTCGGAACCTGTGATCCAAGTCAGGATCGGTAAATAATCAGCAG
TGTTTCCAGCACCAAAACAACCCATTCCCTCTGCTATAAGCTCCCGCACGCGTTTAGCCT
CCGGGTCATCCTCTGCACCATCTCCGTAGTAACATTTTCCAGCCAACATTCTTATAATGT
TGTTGAATGTTAAGTTAGAAAACATTGATTTCATCTCAACCTTTGTAAATTCCTGCAAAA
TTTATAATGCTACATGTAAATATTAAAAATGAGTATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTAT

Last Updated on 06.12.2018 16:11