Sequences from confirmation amplicon


Confirmation sequences for line 081B08 and hit At5g08590 are shown.
Line ID 081B08
Locus At5g08590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2784644
Primers used for PCR TU01 - 8474, show primer details
   
Sequence Name 09-K023622-022-081-B08C-8409
GenBank Accession LN490001 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K023622-022-081-B08C-8409
GTAGATTTCCCGGACATGAAGCCATTTTATTAATTGAAATCAATTGAATACTCATTGCTA
ATCAGTATTTATTCAGATTAGCATGTTCTTTAATCAAAAGTATTATCTATTCACTCCTCT
CTTGATTCTTATATCTTTGTCATGTTTTCAATTTGATTTCTATGGTGTAGTCATCTATAC
TACATTCTAGGCCTAAATCAACTGTTGGAACTCCAGCTTACATAGCACCTGAAGTTCTTT
CACGGAGAGAATATGATGGCAAGGTACAGTATTTTTGTCTTGTTATACATACATATCAGT
TTGTCATTAACGCTTGATTGGTTGTCAAAGTAAGCAAATAATGTCATCAAGGGAAAATAT
GAGGATAGAAAGTTATAGACTGCTCAAAGATCCAGGCTATAGAGTCCTTCTAGTATATCT
TTTTTAGTGGCATGGTCTGTGTGAAACTTTTCAGGTTATTGTACTTCTAATAAGATTTAA
GTGGGGTCTTAATTAAAAAATTTGACGGGGTGCTAATCGATATTTTTTCCATGATTGTCA
TAAGAGCACATANCACTGGTTTTGGCTCTCATATATATGACTGCGTTTTTGTAGCACGCG
GATGTGTGGTCATGTGGAGTAACCCTTTATGTGATGCTGGTGGGAGCTTACCCGTTTGAG
GACCCTAATGATCCAAAAAA
   
Sequence Name 10-K023622-022-081-B08C-TU01
GenBank Accession LN490474 [GenBank]
Sequencing primer gene specific primer, TU01: 5'-CTGGATTGTTTTCCTGAAGTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K023622-022-081-B08C-TU01
ATCATTAGGGTCCTCAAACGGGTAAGCTCCCACCAGCATCACATAAAGGGTTACTCCACA
TGACCACACATCCGCGTGCTACAAAAACGCAGTCATATATATGAGAGCCAAAACCAGTGT
ATGTGCTCTTATGACAATCATGGAAAAAATATCGATTAGCACCCCGTCAAATTTTTTAAT
TAAGACCCCACTTAAATCTTATTAGAAGTACAATAACCTGAAAAGTTTCACACAGACCAT
GCCACTAAAAAAGATATACTAGAAGGACTCTATAGCCTGGATCTTTGAGCAGTCTATAAC
TTTCTATCCTCATATTTTCCCTTGATGACATTATTTGCTTACTTTGACAACCAATCAAGC
GTTAATGACAAACTGATATGTATGTATAACAAGACAAAAATACTGTACCTTGCCATCATA
TTCTCTCCGTGAAAGAACTTCAGGTGCTATGTAAGCTGGAGTTCCAACAGTTGATTTAGG
CCTAGAATGTAGTATAGATGACTACACCATAGAAATCAAATTGAAAACATGACAAAGATA
TAAGAATCAAGAGAGGAGTGAATAGATAATACTTTTGATTAAAGAACATGCTAATCTGAA
TAAATACTGATTAGCAATGAGTATTCAATTGATTTCAATTAATAAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTC

Last Updated on 06.12.2018 16:11