Sequences from confirmation amplicon


Confirmation sequences for line 082E03 and hit At5g64300 are shown.
Line ID 082E03
Locus At5g64300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25720621
Primers used for PCR HM53 - 3144, show primer details
   
Sequence Name 29-K035981-022-082-E03C-3144
GenBank Accession LN499195 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K035981-022-082-E03C-3144
TCCCCTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGTGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTCACAATAAACTATTAGGTAT
GAAGCCATTGTTGGGAGAGTCCCTCTATTGAGTCTTATCACGAAGGAGAATAAGAGATAT
CTGGAGACAAAGCGGACCAAGATGGGTCACATGTATGGCTTGAAGTTCAAAGGGGATGTT
GTGGAGAAGATTGAGTCTGAATCTGAGTCCTAAGCTTAAAAACCAGGACGAACCGAATGG
AATCAAGAACTATAGATATAATACTTCACAAAAAACAAGGAAAGAAATTGACACAGAAGA
AGAGGAAAAAGACATTTGATCTGTCTGAGAAACTTGATTAGATTGGTTTATGTTCTAATC
TAATCTGATTTGATTTTTTTTTATTTTGTCTACGATTCTTGAGTTACGAAATGTTCATCA
TTTGTTACTTCTTGTTGTCGGGATTGAGTAGTTGCATTTTTTTAGTTGTGGAGCTGTGAC
AAAGAGGCCAGCTAGTGTGTGGAATGTGGATCATCATCCTTTGGTTGATTCCTG
   
Sequence Name 30-K035981-022-082-E03C-HM53
GenBank Accession LN499626 [GenBank]
Sequencing primer gene specific primer, HM53: 5'-GGATTCTACAAGGAATCAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K035981-022-082-E03C-HM53
ACTAGCTGGCCTCTTTGTCACAGCTCCACAACTAAAAAAATGCAACTACTCAATCTCGAC
AACAAGAAGTAACAAATGATGAACATTTCGTAACTCAAGAATCGTAGACAAAATAAAAAA
AAATCAAATCAGATTAGATTAGAACATAAACCAATCTAATCAAGTTTCTCAGACAGATCA
AATGTCTTTTTCCTCTTCTTCTGTGTCAATTTCTTTCCTTGTTTTTTGTGAAGTATTATA
TCTATAGTTCTTGATTCCATTCGGTTCGTCCTGGTTTTTAAGCTTAGGACTCAGATTCAG
ACTCAATCTTCTCCACAACATCCCCTTTGAACTTCAAGCCATACATGTGACCCATCTTGG
TCCGCTTTGTCTCCAGATATCTCTTATTCTCCTTCGTGATAAGACTCAATAGAGGGACTC
TCCCAACAATGGCTTCATACCTAATAGTTTATTGTGAACCGAAGGCGGGAAACGACAATC
TGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGT
CTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAT
CCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTG
G
Primers used for PCR MC52 - 3144, show primer details
   
Sequence Name 39-K035982-022-082-E03C-3144
GenBank Accession LN503272 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K035982-022-082-E03C-3144
TCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTCACAATAAACTATTAGG
TATGAAGCCATTGTTGGGAGAGTCCCTCTATTGAGTCTTATCACGAAGGAGAATAAGAGA
TATCTGGAGACAAAGCGGACCAAGATGGGTCACATGTATGGCTTGAAGTTCAAAGGGGAT
GTTGTGGAGAAGATTGAGTCTGAATCTGAGTCCTAAGCTTAAAAACCAGGACGAACCGAA
TGGAATCAAGAACTATAGATATAATACTTCACAAAAAACAAGGAAAGAAATTGACACAGA
AGAAGAGGAAAAAGACATTTGATCTGTCTGAGAAACTTGATTAGATTGGTTTATGTTCTA
ATCTAATCTGATTTGATTTTTTTTTATTTTGTCTACGATTCTTGAGTTACGAAATGTTCA
TCATTTGTTACTTCTTGTTGTCGGGATTGAGTAGTTGCATTTTTTTAGTTGTGGAGCTGT
GACAAAGAGGCCAGCTAGTGTGTGGAATGTGGATCATCATCCTTTGGTT
   
Sequence Name 40-K035982-022-082-E03C-MC52
GenBank Accession LN503628 [GenBank]
Sequencing primer gene specific primer, MC52: 5'-ATTCTACAAGGAATCAACCAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K035982-022-082-E03C-MC52
CTAGCTGGCCTCTTTGTCACAGCTCCCAACTAAAAAAATGCAACTACTCAATCCCGACAA
CAAGAAGTAACAAATGATGAACATTTCGTAACTCAAGAATCGTAGACAAAATAAAAAAAA
ATCAAATCAGATTAGATTAGAACATAAACCAATCTAATCAAGTTTCTCAGACAGATCAAA
TGTCTTTTTCCTCTTCTTCTGTGTCAATTTCTTTCCTTGTTTTTTGTGAAGTATTATATC
TATAGTTCTTGATTCCATTCGGTTCGTCCTGGTTTTTAAGCTTAGGACTCAGATTCAGAC
TCAATCTTCTCCACAACATCCCCTTTGAACTTCAAGCCATACATGTGACCCATCTTGGTC
CGCTTTGTCTCCAGATATCTCTTATTCTCCTTCGTGATAAGACTCAATAGAGGGACTCTC
CCAACAATGGCTTCATACCTAATAGTTTATTGTGAACCGAAGGCGGGAAACGACAATCTG
ATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCT
TGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCC
ACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG
Primers used for PCR 06ax - 8474, show primer details
   
Sequence Name 01-K106867-0022-082-E03C-8409
GenBank Accession LN486593 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K106867-0022-082-E03C-8409
CCAACATACTTTGCGGGATTATTTGTCATCAGCTTCATTGTCCTAACACCTAAATCCCTT
ATTATCTGCAGATTCAAGAAGATTTAGTAAAATACCCGGAAAAAAAATTGTTGTCTTTTT
TGGATGATAATGTGATCTTGGTTAATACCTGTGCACCAATTCCATACTCTCTAGAATCAA
CAGGAAGTCCTAATTCCTCATTAGCTTCAACCGTGTCTCGACCAGCATCTTGCAGATTGT
AAGCTCGAAGCTTGTGTCCTAAACCGATCCCTCTTCCTTCATGTCCACGTAGGTAAACCA
GCACACCGCGACCAGTAGCCTCGATCTGCTGCATCGAGAGTGCTAGCTGGTTCCCGCAAT
CACACCTTGCAGACCCAAATATGTCCCCTGTTAGACATTCAGAATGAACCCTCACGAGAA
TGTCTTGACCGTCACCAATCTCACCCTGCCACAATTTCAGACAAATGGGTTTAGCTTAAG
TCCTTGGATGTTCTGAAGACAAATCAA
   
Sequence Name 11-K106868-0022-082-E03C-06ax
GenBank Accession LN491132 [GenBank]
Sequencing primer gene specific primer, 06ax: 5'-TGATTTGTCTTCAGAACATCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K106868-0022-082-E03C-06ax
GAATTGTGGCGGGTGAGATTGGTGACGGTCAGACATTCTCGTGAGGGTTCATTCTGAATG
TCTAACAGGGGACATATTTGGGTCTGCAAGGTGTGATTGCGGGAACCAGCTAGCACTCTC
GATGCAGCAGATCGAGGCTACTGGTCGCGGTGTGCTGGTTTACCTACGTGGACATGAAGG
AAGAGGGATCGGTTTAGGACACAAGCTTCGAGCTTACAATCTGCAAGATGCTGGTCGAGA
CACGGTTGAAGCTAATGAGGAATTAGGACTTCCTGTTGATTCTAGAGAGTATGGAATTGG
TGCACAGGTATTAACCAAGATCACATTATCATCCAAAAAAGACAACAATTTTTTTTCCGG
GTATTTTACTAAATCTTCTTGAATCTGCAGATAATAAGGGATTTAGGTGTTAGGACAATG
AAGCTGATGACAAATAATCCCGCAAAGTATGTTGGTTTGAAGGGATAAGAGATATCTGGA
GACAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11