Sequences from confirmation amplicon


Confirmation sequences for line 082G09 and hit At3g19080 are shown.
Line ID 082G09
Locus At3g19080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6597745
Primers used for PCR IH26 - 3144, show primer details
   
Sequence Name 77-K100854-0022-082-G09C-3144
GenBank Accession LN513509 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K100854-0022-082-G09C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTATGGTGGATGTGCTAATTCTGTCACCCCAGTAAA
CGCCTGAAGCTCTGGCGAGAGGCTGCAAACTTTAGCAAAGCCGCCTCCTCCTTTTCTCTT
GGGCTTCTCTACCGACTTGGCTGGCCTATTTAATAGAGAAAACTTAGTTTATGGGCACAG
GGTGGCGATAACTAGCTGTACAATTACAAGAACTGAGGGAGAAAGAGAAAACAAACTTTT
TTCTCTTGCGTTTCCGCAAACTCCTAACTTCCTGCTCTTCATTTCCTTCTT
   
Sequence Name 77-K100860-0022-082-G09C-IH26
GenBank Accession LN513510 [GenBank]
Sequencing primer gene specific primer, IH26: 5'-GAAGAAGAAAGTGAAGAGCAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K100860-0022-082-G09C-IH26
AAAGTTTGTTTTCTCTTTCTCCCTCAGTTCTTGTAATTGTACAGCTAGTTATCGCCACCC
TGTGCCCATAAACTAAGTTTTCTCTATTAAATAGGCCAGCCAAGTCGGTAGAGAAGCCCA
AGAGAAAAGGAGGAGGCGGCTTTGCTAAAGTTTGCAGCCTCTCGCCAGAGCTTCAGGCGT
TTACTGGGGTGACAGAATTAGCACATCCACCATACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGG
ATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAATTCAAATCCA

Last Updated on 06.12.2018 16:11