Genotyping and primer details


Genotyping and primer details for 082G09 - At3g19080
Line ID 082G09
Locus At3g19080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6597745 of line 082G09 Chr3:6597745
   
Insertion allele amplicon 3144 - IH26
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IH26: 5'-GAAGAAGAAAGTGAAGAGCAGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6597491
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11