Sequences from confirmation amplicon


Confirmation sequences for line 083F12 and hit At2g24230 are shown.
Line ID 083F12
Locus At2g24230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10303177
Primers used for PCR KE15 - 8474, show primer details
   
Sequence Name 20-K102867-0022-083-F12C-8409
GenBank Accession LN495167 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102867-0022-083-F12C-8409
GTTACTCATAGGAGCATTGATTTTTGTAGCATTTGGCTGCAGAAGGAAAACTAAATCCGG
TGAAGCCAAAGATCTCTCAGTAAAGGAGGAACAAAGCATTTCAGGTCCATTCTCTTTCCA
AACAGATTCCACTACATGGGTGGCTGATGTGAAGCAAGCAAATGCAGTTCCTGTGGTTAT
CTTTGAGAAGCCATTGTTGAACATAACGTTTTCGGATCTCTTGTCTGCAACTTCCAACTT
CGACAGAGACACTCTCTTAGCGGACGGCAAATTTGGTCCTGTTTATCGAGGTTTCCTCCC
CGGTGGGATACATGTAGCTGTAAAAGTTTTGGTTCATGGCTCAACTCTTAGTGACCAAGA
AGCAGCTAGAGAGCTCGAGTTTCTTGGAAGGATCAAGCACCCAAATCTTGTCCCATTAAC
TGGATATTGCATAGCCGGTGATCAAAGGATCGCCATTTACGAGTACATGGAAAACGGTAA
CTTGCAAAACCTGCTTCATGATCTGCCATTTGGAGTTCAGACAACAATGAATTGG
   
Sequence Name 20-K102868-0022-083-F12C-KE15
GenBank Accession LN495168 [GenBank]
Sequencing primer gene specific primer, KE15: 5'-TCCAATCATCTGTTGTCTGAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K102868-0022-083-F12C-KE15
TTTCCTGTACTCGTAAATGGCGATCCTTTGATTCCCTAGAGTGCAATATCCAGTTAATGG
GACAAGATTTGGGCGCTTGATCCTTCCAAGAAACTCGAGCTCTCTAGCTGCTTCTTGGTC
ACTAAGAGTTGAGCCATGAACCAAAACTTTTACAGCTACATGTATCCCACCGGGGAGGAA
ACCTCGATAAACAGGACCAAATTTGCCGTCCGCTAAGAGAGTGTCTCTGTCGAAGTTGGA
AGTTGCAGACAAGAGATCCGAAAACGTTATGTTCAACAATGGCTTCTCAAAGATAACCAC
AGGAACTGCATTTGCTTGCTTCACATCAGCCACCCATGTAGTGGAATCTGTTTGGAAAGA
GAATGGACCTGAAATGCTTTGTTCCTCCTTTACTGAGAGATCTTTGGCTTCACCGGATTT
AGTTTTCCTTCTGCAGCCAAATGCTACAAAAATCAATGCTCCTATGAGTAAACTAATCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAAT
GAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAAT
AAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAATCACGTC
Primers used for PCR KE16 - 3144, show primer details
   
Sequence Name 21-K102867-0022-083-F12C-OriL
GenBank Accession LN495616 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K102867-0022-083-F12C-OriL
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCATTTGAATATTAATCAATTGCTGATTAAGTCCTCCAG
TGACTGATCTTTTTCTCTTGAAAAGTGCTGGGTTTGCAGCGATAGGGCAGCTATTTGTAG
AACCAAAGAAGGATCTGTTCAGAGTTTCAGCTGAGAACTTTCCGGAACAAAATGTTAAGT
TGTTGAAAGAGAAGTTGAATCTCTCCATCCAAGGGAGCTTCTCAAGGATAGACATCGGTA
TCTCTCCCGTCAAATTGTTCCTCGACACATCGATAGCCACAAGGTTCTTGATACTCAAAA
TCGGGATATGTCCTGCAAGATGGTTTCCAGAAACATCGAGTGTACTCAAATCACTCAGCT
TTGAGATCTCTCTAGGTATGTGACCAGAAAGATTTGTGTTTGACAAGTTAAGATACTCTA
AACCAGAAAGCACNTCAATCC
   
Sequence Name 21-K102868-0022-083-F12C-KE16
GenBank Accession LN495617 [GenBank]
Sequencing primer gene specific primer, KE16: 5'-AGGATTGAGATGCTTTCTGGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K102868-0022-083-F12C-KE16
CCAATCTTTCTGGTCCATACCTAGAGAGATCTCAAAGCTGAGTGATTTGACTCAAACGAT
GTTTCTGGAAACCATCTTGCAGGACATATCCCGATTTTGAGTATCAAGAACCTTGTGGCT
ATCGATGTGTCGAGGAACAATTTGACGGGAGAGATACCGATGTCTATCCTTGAGAAGCTC
CCTTGGATGGAGAGATTCAACTTCTCTTTCAACAACTTAACATTTTGTTCCGGAAAGTTC
TCAGCTGAAACTCTGAACAGATCCTTCTTTGGTTCTACAAATAGCTGCCCTATCGCTGCA
AACCCAGCACTTTTCAAGAGAAAAAGATCAGTCACTGGAGGACTTAATCAGCAATTGATT
AATATTCAAATGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCA
CGATGCTCCTCGTGGGTAGGGGTCCATCTTTGGGA

Last Updated on 06.12.2018 16:11