Sequences from confirmation amplicon


Confirmation sequences for line 083G09 and hit At3g21380 are shown.
Line ID 083G09
Locus At3g21380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7528748
Primers used for PCR IH27 - 8474, show primer details
   
Sequence Name 53-K100853-0022-083-G09C-8409
GenBank Accession LN508083 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K100853-0022-083-G09C-8409
GCCATTTACAATTGAATATATACTACATCTTTCATTTATGGATGTTGCAATGGGTTTAGT
TTGGTCGGTCTCGGTCTTGTGGTCTTGACTCTTGACGGGTTGCCTTAGTTTGATTAACTG
ACACTTAATTACTAAATGAAAAGAAAAAATGATGGGTCCTGCTAGGTCTAATAATAGCAT
ATAAAAATGAATTAGGGCTAGGAAAATTTTGGTCTACTATATTTTTGTTTATAATGATTT
ATTTATTGTATACTTTGATCGAACTAATGTAATATATTTGTTTTTGTAGGTGAGCTTGAG
CCCAGACGAGTACATAACAGATGTTACTGGCTACTACAAAACTACAGGGGCTGAGAATGC
TAT
   
Sequence Name 53-K100859-0022-083-G09C-IH27
GenBank Accession LN508084 [GenBank]
Sequencing primer gene specific primer, IH27: 5'-TATAGCATCTTCAGCCCCTGTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K100859-0022-083-G09C-IH27
TCGTCTGGGCTCAAGCTCACCTACAAAACAAATATATTACATTAGTTCGATCAAAGTATA
CAATAAATAAATCATTATAAACAAAAATATAGTAGACCAAAATTTTCCTAGCCCTAATTC
ATTTTTATATGCTATTATTAGACCTAGCAGGACCCATCATTTTTTCTTTTCATTTAGTAA
TTAAGTGTCAGTTAATCAAACTAAGGCAACCCGTCAAGAGTCAAGACCACAAGACCGAGA
CCGACCAAACTAAACCCATTGCAACATCCATAAATTGAAAGATGTAGTATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11