SimpleSearch - Line and FST details
Line specific information
| Line ID | 083G09 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N407953) |
| Segregation Analysis | 50:30:27 |
| Confirmed for Hit | At3g21380 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 96932, 96933 |
Gene hit At5g11425
| Sequence (A. th genome BLAST matches underlined) | >71-011869-strizhov-G9-35St ACTGAACGTAGGGNATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATA AGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGC CTTCGGTTTAAACTATCAGTGTTTGAAAGAAAAAACCCAAAACAAAACTGTAGTCTCGTT GTAACACCAAAACGGTGGCGCTTGCGCACAGTTGTAGTCTTGTAGATATTAAGCTGAGAA GTTATTAAAAATGTAAGTGGGCCTTTTCATCATGATCCAATATATTTTTCGCATAAGCCC AATAGCCCAATATGTGCGATCCACGTCGAGCACAGAGTTTCCTTTTATCACAAAGCAAAG AGAAGAGAGAGCTTCAGAATCATCACAAACGCTAATCCGTCTCATCCGGATTGGTTCCTC TCGTCACCTATGGGATCCTCCCTATAGTGAATCGTATTACTCACGNNNCNGCGAAACGGG GGTGGGCTCCTTTTTCTTTCGCCGGGGCGCCCCGTGTGGGCGGGCCCATATAATAACCTT GGGGAGGACTCCTATGTCCCAGTAGAAACAATCGGATGAGACGGTTAACGTAAGAGGAAA TTTCTGAACCTCTCCTCTCTCTGCTTCCGGAAAAACAGAACTTTTGCTGATTTGGTATCA CATATTGGGCTACTTGGCTTATGCCAAAATATTTGGCTACGTAATAAGAGCCCTTTTATT TTTAAAACTTTAAAATCTATATTACAAAAACAACTTGTGCGAAGGCCCCCGCTTTGGGTG TCCAACACACTACCCTTTTTTTTGGGGGTTTTTTTTTACACCGGAAGGCTAAACCAGGGG GGGAAAAAAATTTTATCCCTAGGACCCTGCTCCTCAAAAAAAGAAACCTCTTTTTAAAAA AAGGCTCTTCAAACAAACTGGCGACCGGCCCCTCTTCAGTGGAGAAAAAAAAAAACCGGG ATTTAAACAATGTTTTTGAAATTCTT |
| GenBank Accession | AL769124 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:3647669 - go to primer design |
| BLAST e Value | 7e-160 |
| Hit Clone Code (BAC ID) | F2I11 |
| Hit Gene Code | At5g11425 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | hypothetical protein |
| Insertion Classification | 3' region |
| Confirmation Status | unknown |
Gene hit At3g21380
| Sequence (A. th genome BLAST matches underlined) | >71-011868-strizhov-G9-8409 TTGATCCATGGTAGATTTCCCGGACAGTGAAGCATTTACAATTGAATATATACTACATCT TTCAATTTATGGATGTTGCAATGGGTTTAGTTTGGTCGGTCTCGGTCTTGTGGTCTTGAC TCTTGACGGGTTGCCTTAGTTTGATTAACTGACACTTAATTACTAAATGAAAAGAAAAAA TGATGGGTCCTGCTATGGGATCCTCCCTATAGTGAGTCGTATTACTCAANNNNNNNNNNG NNNNNNNCNNGNNGNGGGCGNNNGCCCGNGGGGCGACCCGCCGGGCCACACGGCCGCACC ACGCGGGGACCCCCACCGCGGAGGCCACCCGGACCTACCCCCGCAGCGCACCGGGCGACG TACAAACGCCGGGGAACCTCCCCACCCCGCCACCACCCTCCCCAAGCGCCGCGCCACCGC GCCGCCGCGACGGGCAAAGAACCCCATCCCCAGCCTACGGGGCCCGGCCGAGAACCCGCC TGGTCCGAAGTAACACCACGGCCTCCTCGCTGAGGGGGAACTCTTCCACCCTCAGTCTCC TAAACCCCCCCTAGCGGCGCTGTTTAAAAAAAAAAGACAGCCCCACGCTCACCCCTAACC GACGGCACGTCACGGCTACTAATACTCGAAGTCCACGGAGGGTCCTTGGTCGGGGGCGTG GGCCGGCCCGCCCGCCGCCGCTCCCTTGATCCCCACCGGCCCTCAGAACGGGGCGCCGGC GCACGCGGCCGCACGTGAAAACATCCGAAGACACAATACGAGGAAGAAAGAAAACAAAAG AAGGAATGG |
| GenBank Accession | AL769123 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr3:7528748 - go to primer design |
| BLAST e Value | 3e-79 |
| Hit Clone Code (BAC ID) | MHC9 |
| Hit Gene Code | At3g21380 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Mannose-binding lectin superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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