Sequences from confirmation amplicon


Confirmation sequences for line 083H10 and hit At5g01800 are shown.
Line ID 083H10
Locus At5g01800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:308070
Primers used for PCR 09ra - 8474, show primer details
   
Sequence Name 25-K115806-0022-083-H10C-09ra
GenBank Accession LN497598 [GenBank]
Sequencing primer gene specific primer, 09ra: 5'-AACCATTCGGTAAGAACATTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115806-0022-083-H10C-09ra
TATTCAATTTGTTAAAATCTGTTCACTGGATTATTTGAATACGTTACGTTTTCAGAAAGC
ATTCAATCTGATATAGACATCCTGATGTATGTTTTTTTTTTTAATTGGGTTTAAAAAGTT
TGCTCCATATAAAATTTGTGTTGATCTCCTATGTATGATTGTTTCGTTTGCAAAATCACC
TCAGGATGACAACCAAGTCTGTGAACTATGTGACAAGTATGTCACGCTAGTCATTGATTA
CCTTCAAGACTACAACAACCAGAATGAACTCGTTGAGGCCCTTCATATCACCTGCTCTCA
AATTCCTCCTCTCAAAAAACAGGTCTTCATTGAGTCCTTGGTTGTTTTTGCTTTCTCTAT
TCCTTTTCTCAAACGTGGTTATATGTTAACTATGCCCAACTCAGGTGTTTGTTGCAGTGT
CTTTCAATGGTAAATCATTACACCCAACTTTTCTTCACACAAGTCTCTACTATCAAATCA
AACCAAATCTGCAAACGGCTTAACCTCTGTCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCATTTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 04-K115805-0022-083-H10C-8409
GenBank Accession LN488054 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115805-0022-083-H10C-8409
TCGGACTGAGCCTTTCATTGAATATATCCTGACAGAGGTTAAGCCGTTTGCAGATTTGGT
CTGATTTGATAGTAGAGACTTGTGTGAAGAAAAGTTGGGTGTAATGATCTACCATTGAAA
GACACTGCAACAAACACATGAGTTGGGCATAGTTAACATATAACCACGTCTGAGAAAAGG
AATAGAGAAAGCAAAAACAACCAAGGACTCAATGAAGACCTGTTTTTTGAGAGGAGGAAT
CTGAGAGCAGCTGATATGAAGGGCCTCAACGAGTTCATTCTGGTTGTCGTAGTCTTGAAG
GTAATCAATGACTAGCGTGACATACTTGTCACATAGTTCACAGACTTGGTTGTCATCATG
AGCTGATTCTGCAAACGAAACAATCATACATAGGAGATCAACACAAATATTATATGGAGC
AAACTTCTTAAACCCAATTAAAAAAAAAAACATACTTCAGGATGTCTATTTCAAATTGAA
TGCTTTCTGAAAACGTAACGTATTCAAATAATCCAGTGAACAGATTTTAACAAATTTGAA
TTAAAAACAGGTGCAAGAAAGACTCTAGATGCCTTCAAAAATGTTC

Last Updated on 06.12.2018 16:11