Sequences from confirmation amplicon


Confirmation sequences for line 084C05 and hit At1g54020 are shown.
Line ID 084C05
Locus At1g54020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20163503
Primers used for PCR 08cy - 8474, show primer details
   
Sequence Name 29-K109341-0022-084-C05C-08cy
GenBank Accession LN499327 [GenBank]
Sequencing primer gene specific primer, 08cy: 5'-GTTTGCAATATTGAAGTACCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109341-0022-084-C05C-08cy
TTTATTGATTTAGTAAAATATTTAACAACTGTATTGGGACAGAATTTTAACATGAAGGAT
AACAAATAAGCATCAATCCACAAACCATATGTTGTCCTCTATATGTTTACTTGGGATTAG
AAAGATATAATAAAACAATCTGATTCCTTTTGAAAAAATTAAAAGTGTAAAATAGAGATA
AATTTACAAACTTACCAATAAAGTCCGGGACAATTTTGCCATCAGAAAACTTGCCCTTAG
GGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATAATATT
   
Sequence Name 13-K109340-0022-084-C05C-8409
GenBank Accession LN492080 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K109340-0022-084-C05C-8409
ATTTCCCTAAGGGCAAGTTTTCTGATGGCAAAATTGTCCCGGACTTTATTGGTAAGTTTG
TAAATTTATCTCTATTTTACACTTTTAATTTTTTCAAAAGGAATCAGATTGTTTTATTAT
ATCTTTCTAATCCCAAGTAAACATATAGAGGACAACATATGGTTTGTGGATTGATGCTTA
TTTGTTATCCTTCATGTTAAAATTCTGTCCCAATACAGTTGTTAAATATTTTACTTAAAT
CAATAAATGATTTTATAATGAGTTCGATTAAAAATAAGCAATTGCAACGGGTACTTCAAT
ATTGCAAA

Last Updated on 06.12.2018 16:11