Sequences from confirmation amplicon


Confirmation sequences for line 085A06 and hit At5g66700 are shown.
Line ID 085A06
Locus At5g66700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26635052
Primers used for PCR JH03 - 3144, show primer details
   
Sequence Name 01-K019977-022-085-A06C-JH03
GenBank Accession LN486387 [GenBank]
Sequencing primer gene specific primer, JH03: 5'-TCAAATGAGGAGGATATTTTCTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K019977-022-085-A06C-JH03
AGCCCAAATGCAGAGAAGGGTTTTTTACGTGGCATTAAATGAGTGGGCAACTGTAAATGG
GCCACTCATGAGCCCAACTGAGATTGTACGGACGGAACTTTTTTTTTTTTTTTAAGGGGG
GATAAAAATGAAATGACAAAATATCCCCCTTTATTATATTTGGGGGGGGGCCCCAAACAT
GGGTTTAAACCGAAGGGGGGAAACAACAATTTGATCCCCGGGAACCCTGTCCTCTCCAAA
TGAAATGAACTTCCTTATATAAAGGAAGGGTCTTGCAAAGGATAGGGGGATTGGGCGTCA
TCCCTTACGTCAGGGGAAATATCACATCAATCCCCTTGTTTTAAAAACGGGGTTGGAACG
TCTTTTTTTTCCACAATGCTCCTCGGGGGGGGGGGCCCATCTTTGGAAA
   
Sequence Name 05-K019977-022-085-A06C-3144
GenBank Accession LN488161 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K019977-022-085-A06C-3144
GATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTT
CATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAAC
ACATGTTTGGGACCACACCACAAATATAATAAAGTGGGATATTTCGTCATCTCATTCTTA
TCCACCATTAAAAAAAAAAAAAAAGTTCCGTCCGAACAATCTCAGTTGGGCTCATGAGGG
GCCCATTTACAGTTGCCCCCTCTTTTAATGCCCCGTAAAAAACCCTTCTCTGCATTTGGG
CTGACATGCCAAAAAATATCCTCCTCATTTA
Primers used for PCR JH02 - 8474, show primer details
   
Sequence Name 11-K019978-022-085-A06C-JH02
GenBank Accession LN490910 [GenBank]
Sequencing primer gene specific primer, JH02: 5'-GATGTTGAGGAAGAGGAAGTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K019978-022-085-A06C-JH02
CAAGTGAACATGCTCGAATATAGCTTCGGGAACGAGCATAAGCTGGAGTCAGGGAGGAAG
GAGAAGATCGCCGGAGAGTTAGGTCTTGACCCGAGACAGGTGGCTGTTTGGTTCCAGAAC
CGCCGTGCACGTTGGAAAAACAAGAAACTTGAGGGAGAGTACGCCAAACTCAAAAACCAC
CACGACAACGTCGTAATCGGCCAATGCCAA
   
Sequence Name 15-K019978-022-085-A06C-8409
GenBank Accession LN492766 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K019978-022-085-A06C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTAATATAATAAAGT
GGGATATTTCGTCATCTCACATGTGGTGGGATATTTGTAAAATTCGATTCAACTACATTT
CACATATGTGTAATAAATACTCAAAATATACTAATTGACGAATAAACTATATGTTAATTA
TCTAAGAACGGATAAAATTACTCATCTTTCGAAAAAACAAGCAACATGAAATTAAGAATC
ACCAAATTTACTCTGTTTTATAGTGAAAAAACATAAAATTTATACCTGAGACTCGAGTTG
GCATTGGCCGAGTACGACGTTGTCGTGGTGGTTTTTGAGTTTGGCGTACTCTTCCTCTAG
TTTCTTGTTCTTCCAACGTGCACGGCGGTTCTGGAACCAAACAGCCACCTGTCTCGGGTC
AAGACCTAACTCTCCGGCGATCTTCTCCTTCCTCCCTGACTCCAGCTTATGCTCGTTCCC
GAAGCTATATTCGAGCATGTTCACTTGTTCATCGGTCAACTTCCTCTT

Last Updated on 06.12.2018 16:11