Sequences from confirmation amplicon


Confirmation sequences for line 085A11 and hit At4g14230 are shown.
Line ID 085A11
Locus At4g14230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8202619
Primers used for PCR 01lg - 8474, show primer details
   
Sequence Name 42-K114086-0022-085-A11C-01lg
GenBank Accession LN504543 [GenBank]
Sequencing primer gene specific primer, 01lg: 5'-TATGTCTGGTCTCACTTTAGGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114086-0022-085-A11C-01lg
ACGGAGTGGTACTCCAAAGAGAAGAAACAATCTGGTAAATTGATCCTTCTCTTTCGTAAT
TCGTCACCGATCAATTGATTATAAGGAATTGCTTGAATTGATATGAATCTGTATCTGATC
ATTTGACTTTGTGTGAATTTTGCTGTGATTGTGGAATTTGCTTGCGATTGATTGATTTTT
TTTCTCAGCTGCGATTTTTCCGGTTGTTCAGAAACAGCATCAGCTTTTAGTGACTCTGCT
TCTGTTTAATGCTCTTGCTATGGAGGTAATTATCTTCATTCAAAGTCTCTCTGACTTTCT
ATATACTTGCGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATT
   
Sequence Name 66-K114085-0022-085-A11C-8409
GenBank Accession LN511663 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K114085-0022-085-A11C-8409
CGGAAGAAGCCATTTCGCAGTATATAGAAAGTCAGAGAGACTTTGAATGAAGATAATTAC
CTCCATAGCAAGAGCATTAAACAGAAGCAGAGTCACTAAAAGCTGATGCTGTTTCTGAAC
AACCGGAAAAATCGCAGCTGAGAAAAAAAATCAATCAATCGCAAGCAAATTCCACAATCA
CAGCAAAATTCACACAAAGTCAAATGATCAGATACAGATTCATATCAATTCAAGCAATTC
CTTATAATCAATTGATCGGTGACGAATTACGAAAGAGAAGGATCAATTTACCAGATTGTT
TCTTCTCTTTTGGAGTACCACTCCGTTGGAGAATCTCGAGCTCAACGAGACCGAGAGACA
TAAGCCCTAAAGTGAGACCAGACAT

Last Updated on 06.12.2018 16:11