Sequences from confirmation amplicon


Confirmation sequences for line 085B06 and hit At4g17490 are shown.
Line ID 085B06
Locus At4g17490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9753901
Primers used for PCR AB28 - 8474, show primer details
   
Sequence Name 38-K103787-0022-085-B06C-AB28
GenBank Accession LN502952 [GenBank]
Sequencing primer gene specific primer, AB28: 5'-CCTTTGATCCTCGTAGTCTAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103787-0022-085-B06C-AB28
GCTTGATCGCCGTCTCAACGTCCCGAGCCAACACGAGTTCCACGACGAGTCGGGTCTCGA
ATCTCCGCCGCGAATTTCCCCCACGGCCTCATCCTCACTCCTCTGTAATGCCTCTTCTCC
TCTGCTGCTACAACCGGTACGGGAAGTTCCGGTTTAGGATTTCCGGTTGCGAATTGAATC
CACTTGGTTCGGTTCGGTGGTGCGATCTTCAACGGCGGTTTTCGATTGGATTGAACAGTA
ACGCGAGGAGGATTCGATTGGAACGTGAAGGAATCGTTCGATTCAGATGGAATTTCAGAT
TTCACATCGAAATCAAAAATCTCCGGTTTGGGAGTGACGAGATCGATTATTTCCGGTTTG
GTTTCAAATTCAGAAAACTCGAAACCGGTTTGATCAAATGACGTGAAATCGTTCATCCAT
CGATTGGTGGTGGCAGTAGTAGGCAACGGAGACAATTCGTCGAGGAGATACTTCTTGATG
AGGAAAAGAGCTGATACTTCGTTTGGTGTAGCCATTTTGGAGGAAACAGAGAATTGAAGA
GGAAGAGGGATTTGATTTGACAGTGGAGAAGAGAGAGTGGAAAGGTGTATTTATATCCTG
AGGTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTA
   
Sequence Name 54-K103788-0022-085-B06C-8409
GenBank Accession LN508455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K103788-0022-085-B06C-8409
GCCATTTACACCTCAGGATATAAATACACCTTTCCACTCTCTCTTCTCCACTGTCAAATC
AAATCCCTCTTCCTCTTCAATTCTCTGTTTCCTCCAAAATGGCTACACCAAACGAAGTAT
CAGCTCTTTTCCTCATCAAGAAGTATCTCCTCGACGAATTGTCTCCGTTGCCTACTACTG
CCACCACCAATCGATGGATGAACGATTTCACGTCATTTGATCAAACCGGTTTCGAGTTTT
CTGAATTTGAAACCAAACCGGAAATAATCGATCTCGTCACTCCCAAACCGGAGATTTTTG
ATTTCGATGTGAAATCTGAAATTCCATCTGAATCGAACGATTCCTTCACGTTCCAATCGA
ATCCTCCTCGCGTTACTGTTCAATCCAATCGAAAACCGCCGTTGAAGATCGCACCACCGA
ACCGAACCAAGTGGATTCAATTCGCAACCGGAAATCCTAAACCGGAACTTCCCGTACCGG
TTGTAGCAGCAGAGGAGAAGAGGCATTACAGAGGAGTGAGGATGAGGCCGTGGGGGAAAT
TCGCGGCGGAGATTCGAGACCCGACTCGTCGTGGAACTCGTGTTTGGCTCGGGACGTTTG
AGACGGCGATCGAAGCGGCTAGAGCTTACGACAAAGAAGCGTTTAGACTACGAGGATCAA
AGG

Last Updated on 06.12.2018 16:11