Sequences from confirmation amplicon


Confirmation sequences for line 085D04 and hit At3g19940 are shown.
Line ID 085D04
Locus At3g19940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6939579
Primers used for PCR RK39 - 3144, show primer details
   
Sequence Name 49-K015971-022-085-D04C-3144
GenBank Accession LN506661 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K015971-022-085-D04C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAA
CTATCAGTGTTTCCCGTCAATGTTCCGGTTCCTGAGGTTCCGAATCTTGCACCGATGAAA
GAACCAACAAGAAGCTGAAAATAAATAAAGAACATCGGATCATTAAGTCTTTTTGGTGAT
TAGGTATATATGGAATTGGTTTTGTTTCATTTGACTATGGTCATGATTGTTATTTTGCGG
TTCCTTGTAAACCAAGTCATTTTGTTTTACCTGACAAATGAACATTTGAATGCCACCTTC
GAGGAAAAGCAACCTTCTACCATATCTATCAACCGCGTAAATTGAGACAAAGGTTGAAAG
CATGTTGACAACACCGGTTATAACAGCAGACATAAAGGCAGC
   
Sequence Name 50-K015971-022-085-D04C-RK39
GenBank Accession LN507011 [GenBank]
Sequencing primer gene specific primer, RK39: 5'-TGCTGCTCTTATGTCTGCTGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K015971-022-085-D04C-rk39
AACATGCTTTCAACCTTTGTCTCAATTTACGCGGTTGATAGATATGGTAGAAGGTTGCTT
TTCCTCGAAGGTGGCATTCAAATGTTCATTTGTCAGGTAAAACAAAATGACTTGGTTTAC
AAGGAACCGCAAAATAACAATCATGACCATAGTCAAATGAAACAAAACCAATTCCATATA
TACCTAATCACCAAAAAGACTTAATGATCCGATGTTCTTTATTTATTTTCAGCTTCTTGT
TGGTTCTTTCATCGGTGCAAGATTCGGAACCTCAGGAACCGGAACATTGACGGGAAACAC
TGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCC
AAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCG
TCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGA
ACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGG

Last Updated on 06.12.2018 16:11