Sequences from confirmation amplicon


Confirmation sequences for line 085D12 and hit At4g14940 are shown.
Line ID 085D12
Locus At4g14940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8544187
Primers used for PCR PA25 - 8474, show primer details
   
Sequence Name 59-K037020-022-085-D12C-8409
GenBank Accession LN509927 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K037020-022-085-D12C-8409
TCCGGTTATGCCACTCTTCACGGTGGTTTTACTCTTCGGCCTTCAAATTTCTTCGATAAC
GATCCTTTGATTGGGTAAATACGGTCTTAGACAAACAAACTGATGGCTTATGTATTGATT
GACTTATGTTACGTCAAACATATGATCATTGAAAAAACAATTTTTAGCCTTGTGACTAAG
TTATTAATTTGATTTTGATTATTATGTCTTGAATGTATTAATTTCTTATGTTTTGAATGG
TTTAGTTAATTAGCTTTGATGCTTTTTTTGGTTCAATACTTGTGATTCACTTATTTCTCA
ATTTTCAAGTTGGTCGTGCCATCGGCAAAATCCTTTCATTGTAACACCAAAACGTCGATA
AATTTAATGAAATTTGGTTAAATTAGAATCGACCCAAAA
   
Sequence Name 60-K037020-022-085-D12C-PA25
GenBank Accession LN510190 [GenBank]
Sequencing primer gene specific primer, PA25: 5'-TGGGTCGATTCTAATTTAACCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K037020-022-085-D12C-PA25
TTTTGGTGTTACATGAAAGGATTTTGCCGATGGCACGACCAACTTGAAAATTGCGAAATA
AGTGAATCACAAGTATTGAACCAAAAAAAGCATCAAAGCTAATTAACTAAACCATTCAAA
ACATAAGAAATTAATACATTCAAGACATAATAATCAAAATCAAATTAATAACTTAGTCAC
AAGGCTAAAAATTGTTTTTTCAATGATCATATGTTTGACGTAACATAAGTCAATCAATAC
ATAAGCCATCAGTTTGTTTGTCTAAGACCGTATTTACCCAATCAAAGGATCGTTATCGAA
GAAATTTGAAGGCCGAAGAGTAAAACCACCGTGAAGAGTTGGCATAACCGGAAAATCCTC
TATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR PA26 - 3144, show primer details
   
Sequence Name 61-K037020-022-085-D12C-3144
GenBank Accession LN510451 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K037020-022-085-D12C-3144
ATCCCCTATCCTTCGCAAGACCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGATCCATGTAGATTGGTACAACATCACTATGTCTTTGT
TCTCTATCTCTCTGTTCCTGTTCCCATATAACAAACCACAAATTTATTCCTTAATAAACC
AAAAAAAAAAATTACTTACTATGAAAAATAATTTTAAACTTTAAATTAATTATTTTTACT
TTAAATTAATTACCTACTACTCCATACAGCTAAACCGTCATCGCCACGGCTCCAATCACT
ATAAAACCCACCGGCCCATCTCTCGGACCGGTCATAGGCGGTCACCCAAACCGGATATTT
CGTGTAACCGGCTCTTAACTCGGGGTAATCATCATCGGTTAAAAGCGAAGTGGCTTGTAA
ATGTTCCGGTATCAGCCGGTAACCAACTGTATTTCCTATTTTCGTCTTCTTGTTCGGGTT
TACTATTAACAGCTCAACCGGATCCAAGCCAAGTCAAACTCTACCGTCAGCTTCCGTTTT
CCCCGTCTCTTTAACGACCGTCCAGTAACTCTTCCTCCGAGAAAACTTTTGGTTAA
   
Sequence Name 62-K037020-022-085-D12C-PA26
GenBank Accession LN510693 [GenBank]
Sequencing primer gene specific primer, PA26: 5'-AAGTTAACAAAACGTCTTCTCGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K037020-022-085-D12C-PA26
TCGTTAGAGACGGCGAAACGGAAGCTGACGGTAGAGTTCGACTCGGCTCGGATCCGGTTG
AGCTGTTAATAGTAAACCCGAACAAGAAGACGAAAATAGGAAATACAGTTGGTTACCGGC
TGATACCGGAACATTTACAAGCCACTTCGCTTTTAACCGATGATGATTACCCCGAGTTAA
GAGCCGGTTACACGAAATATCCGGTTTGGGTGACCGCCTATGACCGGTCCGAGAGATGGG
CCGGTGGGTTTTATAGTGATCGGAGCCGTGGCGATGACGGTTTAGCTGTATGGAGTAGTA
GGTAATTAATTTAAAGTAAAAATAATTAATCTAAAGTTTAAAATTATTTTTCATAGTAAG
TAATTTTTTTTTTTGGTTTATTAAGGAATAAATTTGTGGTTTGTTATATGGGAACAGGAA
CAGAGAGATAGAGAACAAAGACATAGTGATGTTGTACCAATCTACATGGATCAATCTGAT
CCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTG
CGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
TTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG

Last Updated on 06.12.2018 16:11