SimpleSearch - Line and FST details
Line specific information
| Line ID | 085D12 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N408112) |
| Segregation Analysis | 100:82:39 |
| Confirmed for Hit | At4g14940 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 4005, 89621 |
| Note | |
Gene hit At2g21400
| Sequence (A. th genome BLAST matches underlined) | >92-011849-strizhov-D12-35St TTGACGTAAGGGATGACGCACAAATCCACTATCCTTCGCAAGACCCTTCCTCTATATAAG GAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCT TCGGTTTAAACTATCAGTGTTTGAGTAACTCTTGATGATTATTACTATTATTATTATAAT GATGATCTACCATCACTTTGTGGAGTACTTGATCGAGAAGAATCCCTCTGAAAACATGTC CTCCAATGTTCACCGAGGTCTGATAAGCATATTGCTCTTTTGCATCATCGATTGAACTCA CTTTTACGCATCGGAAATCTGCTATGGGATCCTACCTATAGTGAAGGCGTTATCACTTTA TGGTGAGGCCATTATTTTGCCACTTACAAGACAGACTTGGAGGCCCAAATAGGGCTTTTT GCTTCTTTCTTGCCAACTAACCATTGACAATTCGCCAATCCGAGTGCCATAAGTGCAGAA AAACGCTCTGGGATCCTCCCTATAGAGATCCTCATATAACATATTTGCTGGCCCGTTCGA CCTTCCCATTCAAATTACCCCCTGTCGCTTCCCTATCTTCTCGCACTCGCCCTGGGAACA CTCGCCATACCCCACCCTCCCCCACCCCCCCCTTCTACATCTCTCCTACATTCACCCCTC TCCCTAAACGCTACTCCGCCTCTCGCCCCATTCTCACCCCCCCCGCACCGACTTCCTAGT CCCAACCGCCCCAACTATAAATATCCAAACCCCCACACCCTTCACCTCCTCCAAAAATCC CCATCCTCTCTAATACATTTCCCCTTCCACACACACCCTCATACACCCCCCATCTCATAT ACTGTAAAAATACTACTCCAACTCATACTTTTTCTGCTTACAATTTACTTTTTTCTACT |
| GenBank Accession | AL769320 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:9159447 - go to primer design |
| BLAST e Value | 6e-86 |
| Hit Clone Code (BAC ID) | F3K23 |
| Hit Gene Code | At2g21400 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | SHI-related sequence3 |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At4g14940
| Sequence (A. th genome BLAST matches underlined) | >68-K020160-022-085-D12t-8409 ACCAATGGGAGCCTCCCTATAGTGAGACGGGCATACACCACCAAACTGATGGCTTATGTA TTGATTGACTTATGTTACCTCAAACATATGATCATTGAAAAAACAATTTTTAGCCTTGTG ACTAAGCTATGGGATCCTCCCTAATTATAGTGTCTTGAATGTATTAATTTCTTATGTTTT GAATGGTTTAGTTAATTAGCTATGGGACCCTCCCTATAGCGAGACTTGTGATTCACTTAT TTCTCAATTTTCAAGTTGGTCATGCCATCGGCAAAATCCTTTCATTGTAACACCAAAACG TCTATAAATTTAATGAAATTTGGTTAAATTAGAATCGACCCACACGGTTCTATGGGATCC TCCCTATATTGA |
| GenBank Accession | BX530767 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:8544245 - go to primer design |
| BLAST e Value | 4e-98 |
| Hit Clone Code (BAC ID) | FCAALL |
| Hit Gene Code | At4g14940 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | amine oxidase 1 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | AL769319 [GenBank] BX530768 [GenBank] BX530768 [GenBank] |
Gene hit At3g15010
| Sequence (A. th genome BLAST matches underlined) | >92-011845-strizhov-D12-8409 NTTTGATCCATGGTAGATTTCCCGCACATGAAGCCATTTACAATTGAATATATCCTGTAG CTAACACAGTTCTAGCATGCTGATAAACTAGGAAGAGACACAGGGAAAAGAATAGCAGAG AATTTGTATGGGATCCTCCCTATAGTGAGTCGTATTGCTCAGGGGCACGTCACGGCGGTG GGAGATCGAGCAACGGGTGGAAAACGCACGCAAACTCGGGGGGATAAAACCGACACCTTG AGCTGGACATGCGGCGGGGGAGCCCTTATATCGGGCGGCGCACCTGGTAAGCGTGGAACA CGAGTACAGCGTGGGAGCGAGCCGATACAATCGCGCCGGCGTCTAGGGTACCGGTCCCCC CGCTAGAGGTTGATCCACCCCCTCAGCTGGATATAGCGCAGTCGGCGTTCGAATGATAGT GCATCCGAGTTCTGAAAACGACCTGACTTACAGGCCCTAACTTACGCGACAGGCTGGCGA CCTGCCTTTAACCTGGCGTTTTGTTG |
| GenBank Accession | AL769317 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:5054262 - go to primer design |
| BLAST e Value | 2e-09 |
| Hit Clone Code (BAC ID) | K15M2 |
| Hit Gene Code | At3g15010 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | RNA-binding (RRM/RBD/RNP motifs) family protein |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | failed, line belongs to a contamination group, please check line 085E12 |
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Last Updated on 10.06.2021 13:37 |
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