Sequences from confirmation amplicon


Confirmation sequences for line 085E05 and hit At3g60960 are shown.
Line ID 085E05
Locus At3g60960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22536844
Primers used for PCR BH85 - 8474, show primer details
   
Sequence Name 02-K104526-0022-085-E05C-BH85
GenBank Accession LN487020 [GenBank]
Sequencing primer gene specific primer, BH85: 5'-AAGATTCGATGAAGCTTGTGTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104526-0022-085-E05C-BH85
GTCACGACATTCTAATTCGCGGGTTCTTGGATATAGGGAATTTCGTAAAGGCTAGCCAAA
TCTTCGAGGAATTGAAAGGACTGGATAGTAAACTTCCAGGGAGAGAATATCACAAGGCAA
ATGCAATCTTTAATGTTTCGTTTATGAACTATTGGTTCAAGCAAGGCAAAGATGAGGAAG
CTATGGAGATTCTCGCAAATCTGGAAGATGCTCAAGTGCTGAACCCCATTGTCGGAAACA
GGGTTTTGCAAGTTCTGGTGAAGCACGGTAAAAAAACAGAGGCCTGGGAATTGTTTGGAG
AGATGATAGAAATTTGTGATTCCGAGACTGTTGACATAATGTCCGAGTATTTTAGTGAGA
AGACTGTACCATTCGAGCGGCTTAGGAAAACGTGTTATAGGAAGATGATCGTGAGTCTCT
GCGAGCATGGAAAAGTGTCAGACGCAGAGAAACTCTTCGCCGAGATGTTTACCGATGTTG
ACGGAGGGGACTTGTTGGTGGGGCCTGACCTTTTAATATTCAGAGCAATGATCAATGGTT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGGTTATT
   
Sequence Name 18-K104527-0022-085-E05C-8409
GenBank Accession LN494292 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104527-0022-085-E05C-8409
GCCATTTACACCATTGATCATTGCTCTGAATATTAAAAGGTCAGGCCCCACCAACAAGTC
CCCTCCGTCAACATCGGTAAACATCTCGGCGAAGAGTTTCTCTGCGTCTGACACTTTTCC
ATGCTCGCAGAGACTCACGATCATCTTCCTATAACACGTTTTCCTAAGCCGCTCGAATGG
TACAGTCTTCTCACTAAAATACTCGGACATTATGTCAACAGTCTCGGAATCACAAATTTC
TATCATCTCTCCAAACAATTCCCAGGCCTCTGTTTTTTTACCGTGCTTCACCAGAACTTG
CAAAACCCTGTTTCCGACAATGGGGTTCAGCACTTGAGCATCTTCCAGATTTGCGAGAAT
CTCCATAGCTTCCTCATCTTTGCCTTGCTTGAACCAATAGTTCATAAACGAAACATTAAA
GATTGCATTTGCCTTGTGATATTCTCTCCCTGGAAGTTTACTATCCAGTCCTTTCAATTC
CTCGAAGATTTGGCTAGCCTTTACGAAATTCCCTATATCCAAGAACCCGCGAATTAGAAT
GTCGTAGACCATGAAACTACAACAGCTCATGGATCTCGCCAAAACACAAGCTTCATCGAA
TC

Last Updated on 06.12.2018 16:11