Sequences from confirmation amplicon


Confirmation sequences for line 085G08 and hit At1g05160 are shown.
Line ID 085G08
Locus At1g05160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1488435
Primers used for PCR mr22 - 3144, show primer details
   
Sequence Name 03-K015285-022-085-G08C-3144
GenBank Accession LN487264 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K015285-022-085-G08C-3144
ATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAG
GACAGGGTACCCGGGGATCAGAAATGATCTCTAAAAGAAACCCGGAAAATGGAATTCTTA
TCGCAGGTTAGATTCTGGTCACCTATGAGTATAGCGCAACAGAGATTTTGTATGCGATTT
GTTTTTGGTGATTTTGAAAGAGGTGTTTCTTCACTAGGTTGTCGATGAGACACTTCGAGT
CATAACATTCTCACTCACTGCTTTTCGAGAGGCAAAGACTGACGTTGAAATGAATGGTTA
GTATACATGTAAACACATGACTTATATGAAATACAAAACACCTTCTTAAAGTGCACCTAA
ATATTTAGCATGATTTGATAAATCTTGCAGGCTATTTGATCCCAAAAGGTTGGAAGGTTT
TGACATGGTTTAGGGATGTCCACATCGACCCTGAAGTCTTTCCAGATCCAAGAAA
   
Sequence Name 04-K015285-022-085-G08C-mr22
GenBank Accession LN487707 [GenBank]
Sequencing primer gene specific primer, mr22: 5'-TTTTCTTGGATCTGGAAAGACTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K015285-022-085-G08C-MR22
GGTCGATGTGGACATCCCTAAACCATGTCAAAACCTTCCAACCTTTTGGGATCAAATAGC
CTGCAAGATTTATCAAATCATGCTAAATATTTAGGTGCACTTTAAGAAGGTGTTTTGTAT
TTCATATAAGTCATGTGTTTACATGTATACTAACCATTCATTTCAACGTCAGTCTTTGCC
TCTCGAAAAGCAGTGAGTGAGAATGTTATGACTCGAAGTGTCTCATCGACAACCTAGTGA
AGAAACACCTCTTTCAAAATCACCAAAAACAAATCGCATACAAAATCTCTGTTGCGCTAT
ACTCATAGGTGACCAGAATCTAACCTGCGATAAGAATTCCATTTTCCGGGTTTCTTTTAG
AGATCATTTCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATA
GAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
TCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTC
CTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTCAACGATGGCC
TTTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTCCTTTTCCACTATCTTCACA
ATAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCGGATATTACCCTTTGT
TGAAAAGTCTCAATTGCCCT
Primers used for PCR FI37 - 3144, show primer details
   
Sequence Name 05-K028243-022-085-G08C-3144
GenBank Accession LN488217 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K028243-022-085-G08C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGAAATGATCTCTAAAAGAAACCCGGAAA
ATGGAATTCTTATCGCAGGTTAGATTCTGGTCACCTATGAGTATAGCGCAACAGAGATTT
TGTATGCGATTTGTTTTTGGTGATTTTGAAAGAGGTGTTTCTTCACTAGGTTGTCGATGA
GACACTTCGAGTCATAACATTCTCACTCACTGCTTTTCGAGAGGCAAAGACTGACGTTGA
AATGAATGGTTAGTATACATGTAAACACATGACTTATATGAAATACAAAACACCTTCTTA
AAGTGCACCTAAATATTTAGCATGATTTGATAAATCTTGCAGGCTATTTGATCCCAAAAG
GTTGGAAGGTTTTGACATGGTTTAGGGATGTCCACATCGACCCTGAAGTCTTTCCAGATC
CAAGAA
   
Sequence Name 06-K028243-022-085-G08C-FI37
GenBank Accession LN488669 [GenBank]
Sequencing primer gene specific primer, FI37: 5'-GATCAAATTTTCTTGGATCTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K028243-022-085-G08C-FI37
GGTCGATGTGGACATCCCTAAACCATGTCAAAACCTTCCAACCTTTTGGGATCAAATAGC
CTGCAAGATTTATCAAATCATGCTAAATATTTAGGTGCACTTTAAGAAGGTGTTTTGTAT
TTCATATAAGTCATGTGTTTACATGTATACTAACCATTCATTTCAACGTCAGTCTTTGCC
TCTCGAAAAGCAGTGAGTGAGAATGTTATGACTCGAAGTGTCTCATCGACAACCTAGTGA
AGAAACACCTCTTTCAAAATCACCAAAAACAAATCGCATACAAAATCTCTGTTGCGCTAT
ACTCATAGGTGACCAGAATCTAACCTGCGATAAGAATTCCATTTTCCGGGTTTCTTTTAG
AGATCATTTCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATA
GAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
TCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTC
CTCGTGGGTGGG
Primers used for PCR 01em - 3144, show primer details
   
Sequence Name 25-K110827-0022-085-G08C-01em
GenBank Accession LN497497 [GenBank]
Sequencing primer gene specific primer, 01em: 5'-GATCAAATTTTCTTGGATCTGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K110827-0022-085-G08C-01em
ACCTTCCACCTTTTGGGATCAAATAGCCTGCAAGATTTATCAAATCATGCTAAATATTTA
GGTGCACTTTAAGAAGGTGTTTTGTATTTCATATAAGTCATGTGTTTACATGTATACTAA
CCATTCATTTCAACGTCAGTCTTTGCCTCTCGAAAAGCAGTGAGTGAGAATGTTATGACT
CGAAGTGTCTCATCGACAACCTAGTGAAGAAACACCTCTTTCAAAATCACCAAAAACAAA
TCGCATACAAAATCTCTGTTGCGCTATACTCATAGGTGACCAGAATCTAACCTGCGATAA
GAATTCCATTTTCCGGGTTTCTTTTAGAGATCATTTCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 09-K110826-0022-085-G08C-3144
GenBank Accession LN490235 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K110826-0022-085-G08C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGCGGTA
TAGGGTACCCGGGGATCAGAAATGATCTCTAAAAGAAACCCGGAAAATGGAATTCTTATC
GCAGGTTAGATTCTGGTCACCTATGAGTATAGCGCAACAGAGATTTTGTATGCGATTTGT
TTTTGGTGATTTTGAAAGAGGTGTTTCTTCACTAGGTTGTCGATGAGACACTTCGAGTCA
TAACATTCTCACTCACTGCTTTTCGAGAGGCAAAGACTGACGTTGAAATGAATGGTTAGT
ATACATGTAAACACATGACTTATATGAAATACAAAACACCTTCTTAAAGTGCACCTAAAT
ATTTAGCATGATTTGATAAATCTTGCAGGCTATTTGATCCCAAAAGGTTGGAAGGTTTTG
ACATGGTTTAGGGATGTCCACATCGACCCTGAAGTCTTTCCAGATCCAAGAAAATTTGAT
Primers used for PCR 82wb - 3144, show primer details
   
Sequence Name 38-K118301-0022-085-G08C-82wb
GenBank Accession LN503147 [GenBank]
Sequencing primer gene specific primer, 82wb: 5'-TGAGAGTGAGAATGTAATGGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K118301-0022-085-G08C-82wb
GCTCTTACTTGGGGTTCAGCATGGCAGTCATATTCCTGGATTTGCTTATCATAGAGCACT
CAAGGTCTAAATTAAACCACCCTTTTTTACTAAATTCAATATTCTTTCTTTTGGAAGATT
TTGAAGATCATTATTATTTATTTTGCAGGCGAGGAAAACACTTGTAGCTGCCTTTCAGTC
CATAGTGACTGAGCGCAGAAATCAAAGGAAGCAGAACATTTTATCAAATAAGAAAGATAT
GTTAGACAATCTTCTTAATGTTAAAGATGAAGATGGAAAAACTTTGGATGACGAAGAGAT
TATCGATGTTCTTCTGATGTATCTTAATGCCGGTCATGAATCCTCTGGCCACACCATTAT
GTGGGCTACCGTTTTTCTACAAGAACACCCTGAGGTTCTACAAAGAGCAAAGGTAAGTGG
AGAGAAAAAAACGATTTCATTTCCTTATCATCATCTTATACTTTCTTGTGTTTTGCATAT
GATGGTACAATAGGCTGAACAAGAAATGATCCTGAAAAGTAGGCCTGAAGGTCAAAAAAA
TATTCAAAAAGAAAGAGTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAATCCAATC
C
   
Sequence Name 06-K118300-0022-085-G08C-35St
GenBank Accession LN489008 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K118300-0022-085-G08C-35St
ATCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAC
TCTTTCTTTTTGAATATTTTTTTGACCTTCAGGCCTACTTTTCAGGATCATTTCTTGTTC
AGCCTATTGTACCATCATATGCAAAACACAAGAAAGTATAAGATGATGATAAGGAAATGA
AATCGTTTTTTTCTCTCCACTTACCTTTGCTCTTTGTAGAACCTCAGGGTGTTCTTGTAG
AAAAACGGTAGCCCACATAATGGTGTGGCCAGAGGATTCATGACCGGCATTAAGATACAT
CAGAAGAACATCGATAATCTCTTCGTCATCCAAAGTTTTTCCATCTTCATCTTTAACATT
AAGAAGATTGTCTAACATATCTTTCTTATTTGATAAAATGTTCTGCTTCCTTTGATTTCT
GCGCTCAGTCACTATGGACTGAAAGGCAGCTACAAGTGTTTTCCTCGCCTGCAAAATAAA
TAATAATGATCTTCAAAATCTTCCAAAAGAAAGAATATTGAATTTAGTAAAAAAGGGTGG
TTTAATTTAGACCTTGAGTGCTCTATGATAAGCAAATCCAGGAATATTGACTGCCATTGC
TCGAACCCCATAGTTAAGAGCTGTATATTCTCGTTCCAATGCATCCNTTACATTTTCCAC
TC

Last Updated on 06.12.2018 16:11