Sequences from confirmation amplicon


Confirmation sequences for line 085G10 and hit At1g74690 are shown.
Line ID 085G10
Locus At1g74690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28063126
Primers used for PCR 13wq - 3144, show primer details
   
Sequence Name 17-K118953-0022-085-G10C-13wq
GenBank Accession LN494081 [GenBank]
Sequencing primer gene specific primer, 13wq: 5'-GAACTTTACGGACACTCTTCTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K118953-0022-085-G10C-13wq
TTTTCTGCTTATCGTTTTCTTCGGTTGAGGAACTGGTTTCCAGAAGCATGAGGCTGACCA
GTTCTCTAACCAGATCAAGTTTGAGTTTGGATTGGATGTATCATATGCGTGTACAGGTAG
CACTTTTGGTGATGAAGCGAGAAGCTGTAAACAAAATTATCCATCAAGATCTTTAAATAA
AATTAGAGACTCCATAGTATAAAAGAGATATTGGATACAGTGAACATCATTATAATAAAT
AACTCTACCAACAAGCGTACCTTCTGAGCAAAAGCATTGGCTGTTAGTTTCTTGATTCCA
AGGTAAGCATCTGTAGGATTTGCAAGCTTGTTCCCCTGATAAAAAAAGCCAGTTCTAACA
ATTATTTCCCCACCAACCCAAACTAGAATATCTTCCTCGACTAAGTTTGTATTTAAGTGT
AAGTTTGAACTTACAAAGAAATGAAAGTATCATTACCTGAAGTAGCTGCAATCTGCATTT
CCTATAAACTTGAACTCCAATGTCGGACTTCCTGATCTCTCTTCCTGATAGTTTAAACCG
AAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTT
CCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTAA
GTGGAGATATCAC
   
Sequence Name 01-K118952-0022-085-G10C-35St
GenBank Accession LN486799 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118952-0022-085-G10C-35St
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGGAAGAGAGATCAGGAAG
TCCGACATTGGAGTTCAAGTTTATAGGAAATGCAGATTGCAGCTACTTCAGGTAATGATA
CTTTCATTTCTTTGTAAGTTCAAACTTACACTTAAATACAAACTTAGTCGAGGAAGATAT
TCTAGTTTGGGTTGGTGGGGAAATAATTGTTAGAACTGGCTTTTTTTATCAGGGGAACAA
GCTTGCAAATCCTACAGATGCTTACCTTGGAATCAAGAAACTAACAGCCAATGCTTTTGC
TCAGAAGGTACGCTTGTTGGTAGAGTTATTTATTATAATGATGTTCACTGTATCCAATAT
CTCTTTTATACTATGGAGTCTCTAATTTTATTTAAAGATCTTGATGGATAATTTTGTTTA
CAGCTTCTCGCTTCATCACCAAAAGTGCTACCTGTACACGCATATGATACATCCAATCCA
AACTCAAACTTGATCTGGTTAGAGAACTGGTCAGCCTCATGCTTCTGGAAACCAGTTCCT
CAACCGAAGAAAACGATNAGCAGAAAACCTCAGAACAGGCTTTTAGTCGAGGCTGAGTCT
GCAAAACCCAAGAAGAGTGTCCGTAAAGTT

Last Updated on 06.12.2018 16:11