SimpleSearch - Line and FST details
Line specific information
| Line ID | 085G10 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N408146) |
| Segregation Analysis | 50:24:15 |
| Confirmed for Hit | At1g74690 |
| Parent of DUPLO pair | 578 |
| Parent of pair(s) | none |
| Note | |
Gene hit At3g46010
| Sequence (A. th genome BLAST matches underlined) | >79-011847-strizhov-G10-35St TTGACGTAAGGGATGACGCACATATCCCCTATCCTTCGCAAGACCCTTCCTCTATATAAG GAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCT TCGGTTTAAACTATCAGTGTTAGACAGGATGTATTGGCGGGTAAACCTAAGAGAAAAGAG CGTTTATTAAAATAATCTGATATTTAAAAGGGCGCGAAAATGTTTATCCGTTCGTCCATT TGTATGTGCATGCCAACCACAGGGTTGCCCTCGGGAGTGCTTGGCATTCCGTGCGATAAT GACTTCTGTTCAACCACCCAAACGTCGGAAAGCCTGACGACGGAGCAGCATTCCAAAAAG ATTCCTTGGCTCGTCTGGGTCGGCTAGCTACCACTCAATGAAGAAGATCTTGCTCTTCTG GCAATTCTCAGCAGTTACAAAGTCAAAATCGTAAATGGCGTAACGGCATTCATCATCTGG AAGACATGCTGCATACTCCTCGTAAGTTTGGATCGGTTGACCAACTTTCTCAACAACAAC TTTGCTTCTGCTTCTTCTCAATTTTGTAAACTATGAAACCGTGTGCCCTTTTTACCCTTC AATTCCAAGAAATCTTAAGATAGCAATTCTGTACGGCCAAGCCCTTCCAAAAGGTCCGGT TTGGTCTGTTAAACCAAAAAAACTCTCATTTTCAAATTTTTTTTAAATTAAACCCATTCC TTAACCCTTCTATTGTACTCCAAACCTTTTAAGGTAAATACCTTTATTTTTAAAAAATTT AATTT |
| GenBank Accession | AL769391 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:16909926 - go to primer design |
| BLAST e Value | 1e-90 |
| Hit Clone Code (BAC ID) | F16L2 |
| Hit Gene Code | At3g46010 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | actin depolymerizing factor 1 |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At1g74690
| Sequence (A. th genome BLAST matches underlined) | >79-011846-strizhov-G10-35St CTGACGTAAGGGATGACNGCACAATCCCCTATCCTTCGCAAGACCCTTCCTCTATATAAG GAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCT TCGGTTTAAACTATCAGGAAGAGAGATCATGAAGTGCGACATTGGAGTTCAAGATTATAG GAAATGCAAATTGCAGCTACTTCAGGTAATGATACTTTCATGTCTTTGCAAGCTCACACT TACACTTACATAGCAAACTTAATCGAGGAAGATATTCTATGGGATCCTCCCTATGAGAGA GTGGCATTAGTCAAACCACCCAAACGTCGGAAAGCCTGACGACGGAGCAGCATTCCAAAA AGATCCCTTGGCTCGTCTGGGTCGGCTATGGGATCCTCCCTATAGTGAGTCGTATTACTC |
| GenBank Accession | AL769390 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:28063126 - go to primer design |
| BLAST e Value | 4e-47 |
| Hit Clone Code (BAC ID) | F1M20 |
| Hit Gene Code | At1g74690 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | IQ-domain 31 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At5g38430
| Sequence (A. th genome BLAST matches underlined) | >79-011845-strizhov-G10-8409 NTGATCCATGGTAGATTTCCCGGACCATGAAGCCTTTACAATTGAATATATCCTGTGACT ACAGTCAGCCGTGCTTCTACGGTGCAATCGGCCGCGGTGGCTCCATTCGGCGGCCTCAAA TCCATGACTGGATTCCCAGTTAAGAAGGTCAACACTGACATTACTTCCATTACAAGCAAT GGTGGAAGAGTAAAGTGCATGGTGACGGTGTTCGGCATTCTGAATCTCACCGAGGACTCC TTCTTCGATGAGAGCCGGCGGCTATGGGATCCTCCCTATAGTGAGTCGATNTACTCAANN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAGAN NNNNNNNCNNNNNNNANNGANNCANNNNNCCCNCNNAANNNAACAAAGAGGCCCCACCAA AAAACACAAAGCCACACCCCCAACCCNCTACACACAAGGCGAGGAAACGGAAAGAGCGGC ACGGGCCGGACCTGTGAAACCGGGGAAACCACGGCAAGGCATTGGAAGGGCGCCCAANGG GAGCCCAACGCGCAAAAACCCGCCCCGGGACGTCAAAAAAAAAAAAAAGGGGGNAAAAGG GGCAAAGGCGCGGGGAACAACCACGGGAAAACAAAAGGAGGGACGGGTCGGGGAACGTTG GCAGAAGAAAGAACAAAGTTCTCCCACTCTTTTTTGTTGTT |
| GenBank Accession | FR802534 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:15385032 - go to primer design |
| BLAST e Value | 6e-06 |
| Hit Clone Code (BAC ID) | MXI10 |
| Hit Gene Code | At5g38430 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Ribulose bisphosphate carboxylase (small chain) family protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
|
Last Updated on 10.06.2021 13:37 |
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