Sequences from confirmation amplicon


Confirmation sequences for line 085H02 and hit At1g30990 are shown.
Line ID 085H02
Locus At1g30990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11052831
Primers used for PCR BH84 - 8474, show primer details
   
Sequence Name 45-K104418-0022-085-H02C-BH84
GenBank Accession LN505521 [GenBank]
Sequencing primer gene specific primer, BH84: 5'-CTCCATCAAGACATGGAAGTACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K104418-0022-085-H02C-BH84
TGTATAACTATTCCATTTATTCCTAAAACATTATTTTCTAAACCTTTTTGTTCTACAAAA
ACTGATTTTCTAAATGTCTTTAATTAATTCTATGTTAATAATTTGTAAACATAAAAAAAT
TCTCATTGAAAATATTTTAATTTATGAATGGTTATTGGTCCATAATTATATAATATTTTT
TATTGAGAGTAAATAATGTATTTAAAATAAGTAAACACTAATCAGTGTTTCTTTTTCTTT
TTGTGTAAAAAGTCCTAAATAATATCAATCGGTTAGGAAGAGAGTACATGTGCTGCATTC
ATGAGTATCCACATATCAATCACTAGTCAGTGCGCTGCATTCATGGTATCCACATTACAA
ATGATTTGTTTTAATATGGAAAAAGTAGGGTTTATTTAGTATAGTAAGAAATGAATGTGT
TTGGTCATCATTCATTATATATGAAGATGGGAAGGAGGAAGAGTTTAAGGAGAGAACAGA
GATAGACCCCGCCTGATATTACACCTTAAGATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 61-K104419-0022-085-H02C-8409
GenBank Accession LN510512 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K104419-0022-085-H02C-8409
AATATCAGGCGGGGNTCTATCTCGTGNTTCTCTCCTTAAACTCTTCCTCCTTCCCATCTT
CATATATAATGAATGATGACCAAACACATTCATTTCTTACTATACTAAATAAACCCTACT
TTTTCCATATTAAAACAAATCATTTGTAATGTGGATACCATGAATGCAGCGCACTGACTA
GTGATTGATATGTGGATACTCATGAATGCAGCACATGTACTCTCTTCCTAACCGATTGAT
ATTATTTAGGACTTTTTACACAAAAAGAAAAAGAAACACTGATTAGTGTTTACTTATTTT
AAATACATTATTTACTCTCAATAAAAAATATTATATAATTATGGACCAATAACCATTCAT
AAATTAAAATATTTTCAATGAGAATTTTTTTATGTTTACAAATTATTAACATAGAATTAA
TTAAAGACATTTAAAAAATCATTTTTTGTAAAACAAAAAGGTTTAGAAAATAATGTTTTA
GGAATAAATGGAATAGTTATACATATATGAGATAATGAGTAGAAATACGAACCAAGATTG
TACTTCCATGTCTTGATGG

Last Updated on 06.12.2018 16:11