Sequences from confirmation amplicon


Confirmation sequences for line 085H10 and hit At1g13245 are shown.
Line ID 085H10
Locus At1g13245 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4525990
Primers used for PCR 10lm - 3144, show primer details
   
Sequence Name 50-K114129-0022-085-H10C-10lm
GenBank Accession LN507244 [GenBank]
Sequencing primer gene specific primer, 10lm: 5'-GCTCCTAATTCCCAGAGAGAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K114129-0022-085-H10C-10lm
AAGAAGAAACCCAGTTTCGTCTGTTTGAAAATCCTCGTAGTAATGAGAGACTTGCAACTT
CTTATATATAAAAAATAAATTAGAATTACAACCAAAAAATTAAAATAAATCAACCACGTG
GCGTGCATGTGTGCCATGATACATACCAACAACGGGGACAAATAGGGCATAGATGTTTGG
GGGGTTTTGTGAAATTGTGACCATATAAATCATTCAATATTCAATGATAATAAATTGCAA
TAACGCAAAAAACACACAAGTGGGTAGTGAATAAGAAGTGTGTAGGGCCACGTTTCAATA
TTTATCTCCTTCTATAAAAAGGACTCTCTTCTTCTTTGTATCCTCATTTTCATTAATCCT
TCAATACATCTCTCAAACCCTAAGAGAAGAAAAATTTGCTTTGTTTTTGCCAAATTCAAT
CCAAATTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGT
ACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGAT
AGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
   
Sequence Name 08-K114128-0022-085-H10C-3144
GenBank Accession LN489817 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K114128-0022-085-H10C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAT
TTGGATTGAATTTGGCAAAAACAAAGCAAATTTTTCTTCTCTTAGGGTTTGAGAGATGTA
TTGAAGGATTAATGAAAATGAGGATACAAAGAAGAAGAGAGTCCTTTTTATAGAAGGAGA
TAAATATTGAAACGTGGCCCTACACACTTCTTATTCACTACCCACTTGTGTGTTTTTTGC
GTTATTGCAATTTATTATCATTGAATATTGAATGATTTATATGGTCACAATTTCACAAAA
CCCCCCAAACATCTATGCCCTATTTGTCCCCGTTGTTGGTATGTATCANGGCACACATGC
ACGCCACGTGGTTGATTTATTTTAATTTTTTGGTTGTAATTCTAATTTATTTTTTATATA
TAAGAAGTTGCAAGTCTCTCATTACTACGAGGATTTTTCAAACAGACGAAACTGGGTTGT
CTTCTTCCACCTATAGATGTTATATTGCTTCTAGATAATTATTTTCTCTCTGGGATTTAG
GAG
Primers used for PCR 15ml - 3144, show primer details
   
Sequence Name 33-K114144-0022-085-H10C-15ml
GenBank Accession LN501081 [GenBank]
Sequencing primer gene specific primer, 15ml: 5'-TCTATAGGTGGAAGAAGACAACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K114144-0022-085-H10C-15ml
ATGAGAGACTTGCACTTCTTATATATAAAAAATAAATTAGAATTACAACCAAAAAATTAA
AATAAATCAACCACGTGGCGTGCATGTGTGCCATGATACATACCAACAACGGGGACAAAT
AGGGCATAGATGTTTGGGGGGTTTTGTGAAATTGTGACCATATAAATCATTCAATATTCA
ATGATAATAAATTGCAATAACGCAAAAAACACACAAGTGGGTAGTGAATAAGAAGTGTGT
AGGGCCACGTTTCAATATTTATCTCCTTCTATAAAAAGGACTCTCTTCTTCTTTGTATCC
TCATTTTCATTAATCCTTCAATACATCTCTCAAACCCTAAGAGAAGAAAAATTTGCTTTG
TTTTTGCCAAATTCAATCCAAATTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGA
CAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGA
AGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA

Last Updated on 06.12.2018 16:11