Sequences from confirmation amplicon


Confirmation sequences for line 086A07 and hit At5g57800 are shown.
Line ID 086A07
Locus At5g57800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23421044
Primers used for PCR FI39 - 8474, show primer details
   
Sequence Name 20-K028244-022-086-A07C-FI39
GenBank Accession LN495074 [GenBank]
Sequencing primer gene specific primer, FI39: 5'-CAAATTTCTTTTTCTCAGGATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K028244-022-086-A07C-FI39
AAAACTGTAGAAATCTTTAAGGTTTTTTTACACCAATAAGAGTCAGAGAGAGAGAGAGAG
AGAGAAAGAGAGATCTATTCATAAAAAAGACGGGTAATCTCAACTCCGAGGAGATAACTG
ATATAACAATTTGAGAGAATAAGAAGAAGAAGAAGAAGAAGACCAAGCTAAAGAGTGGTT
GCTTTTTTATCAGCTTGGCCTTGGGAAAACTTTGGCAATCTCAGGGTTTGTCTTTAATCT
TTGGTTTGAGTTTTGAAAGATTTGGTTTCTCTTCTGAATCTCATATGGGTTTTGTGATTT
TGTTGTCTATATGTGAAGTATCTTCTCTACGCTCCATTAGCTGCACAAGTAGTGTACTCG
TGGGTCTATGAAGAAGATATCTCAAAGGTTCTTTGGTGTATTCATATTCTCATAATCTGC
GGTCTCAAAGCACTTGTTCATGAACTATGGAGCGTTTTCAACAACATGCTTTTCGTGACT
CGTACTCTAAGGATTAACCCTAAGGGGATCGACTTTAAACAGATTGATCACGAGGGGCAC
TGGTAAAATCTCCAATCTCATTGACCCATTTCAAAATAATTCATTAAAGTTTTATCATTT
TCCTTTAGCCCTCTGTTTTTCCTCTGTTTTTCCTCTGTTTTGAANAAAAGGATATATTCA
ATTGTAAATGGCTTCGTGTCCGGGAAATCTACGTGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
   
Sequence Name 19-K028244-022-086-A07C-8409
GenBank Accession LN494621 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K028244-022-086-A07C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTTTATTCAAA
ACAGAGGAAAAACAGAGGAAAAACAGAGGACTAAAGGAAAATGATAAAACTTTAATGAAT
TATTTTGAAATGGGTCAATGAGATTGGAGATTTTACCAGTGCCATTCGTGATCAATCTGT
TTAAAGTCGATCCCTTTAGGGTTAATCCTTAGAGTACGAGTCACGAAAAGCATGTTGTTG
AAAACGCTCCATAGTTCATGAACAAGTGCTTTGAGACCGCAGATTATGAGAATATGAATA
CACCAAAGAACCTTTGAGATATCTTCTTCATAGACCCACGAGTACACTACTTGTGCAGCT
AATGGAGCGTAGAGAAGATACTTCACATATAGACAACAAAATCACAAAACCCATATGAGA
TTCAGAAGAGAAACCAAATCTTTCAAAACTCAAACCAAAGATTAAAGACAAACCTTGAGA
TTGCCAAAGTTTTCCCAAGGCCAAGCTGATAAAAAAGCAACCATTCTTTAGCTTGGTCTT
CTTCTTCTTCTTCTTCTTATTCTCTCAAATTGTTATATCAGTTATCTCCTCGGAGTTGAG
ATTACCTGTCTTTTTTATGAATAGATCTCTCTTTCTCTCTCTCTCTCTCTCTCTGACTCT
TATTGGTGTAAAAAAACCTTAAAGATTTCTACAGTTTTCAAGCAATCCT

Last Updated on 06.12.2018 16:11