Genotyping and primer details

Genotyping and primer details for 086A07 - At5g57800
Line ID 086A07
Locus At5g57800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23421044 of line 086A07 Chr5:23421044
Insertion allele amplicon 8474 - FI39
Locus specific primer FI39: 5'-CAAATTTCTTTTTCTCAGGATTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23420388
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11