Sequences from confirmation amplicon


Confirmation sequences for line 086B01 and hit At4g39150 are shown.
Line ID 086B01
Locus At4g39150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18234796
Primers used for PCR 05lj - 8474, show primer details
   
Sequence Name 41-K114144-0022-086-B01C-05lj
GenBank Accession LN504162 [GenBank]
Sequencing primer gene specific primer, 05lj: 5'-TTGATTGGATGATATTCTGGATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114144-0022-086-B01C-05lj
TGATGAGATACTAGGTGAAGCTTATCAAGTTCTAGGCGATCCGGAGAAGCGTACAGCTTA
TGACAAATATGGAAAAGAAGGGGTTCAACAGTAAGTTTTCACACTTTCATTCCTCATGAT
TCATTCTACATTTGAAGATTTTCGGTTTTCCCCACATAAAAAGCCTAGAGATAACTTCTG
TGATGTTTATATGTCATTTGAGAATGTGATTGGTATAATTGCTATAGTATGTTAATTTCA
CTTCCATTTTCTTGCAGGGATGCAATGGTTGATCCTGCAGCTGTGTTTGGTATGCTCTTT
GGTAGCGAGCTCTTTGAGGATTACGTTGGGCAACTTGCCCTTGCTTCAGCAGCCTCAATC
GATGCTGAATTAGAATCGTATGAGCCTGAGATTAGAAAACAGATGCTTCAGGAAAAGATC
AAAGTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA
   
Sequence Name 14-K114143-0022-086-B01C-oriL
GenBank Accession LN492596 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K114143-0022-086-B01C-oriL
TTACATTGAATATATCCTACTTTGATCTTTTCCTGAAGCATCTGTTTTCTAATCTCAGGC
TCATACGATTCTAATTCAGCATCGATTGAGGCTGCTGAAGCAAGGGCAAGTTGCCCAACG
TAATCCTCAAAGAGCTCGCTACCAAAGAGCATACCAAACACAGCTGCAGGATCAACCATT
GCATCCCTGCAAGAAAATGGAAGTGAAATTAACATACTATAGCAATTATACCAATCACAT
TCTCAAATGACATATAAACATCACAGAAGTTATCTCTAGGCTTTTTATGTGGGGAAAACC
GAAAATCTTCAAATGTAGAATGAATCATGAGGAATGAAAGTGTGAAAACTTACTGTTGAA
CCCCTTCTTTTCCATATTTGTCATAAGCTGTACGCTTCTCCGGATCGCCTAGAACTTGAT
AAGCTTCACCTAGTATCTTCATTCACACAACACCAATTTATAGGTTAGAGTGATCCAGAA
TATCTCCCAATCA

Last Updated on 06.12.2018 16:11