Sequences from confirmation amplicon


Confirmation sequences for line 086D06 and hit At1g17150 are shown.
Line ID 086D06
Locus At1g17150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5866196
Primers used for PCR 20jx - 8474, show primer details
   
Sequence Name 69-K113379-0022-086-D06C-20jx
GenBank Accession LN512243 [GenBank]
Sequencing primer gene specific primer, 20jx: 5'-AGGTGACGGTAAAATGGATAACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113379-0022-086-D06C-20jx
TATTCTTTCTCACTTCTTCATTCATTTCACATAGAATGTCGATTGACTCAATAAAAAAAA
AAAATAGGAATAAACCTTTTATTTCCAAAAAAAAAAAAAAACGGGCTATTTACTTGCTTT
TTAATATTGGGATTAATTAAAATTAACTAGTTCATTTTACCTCCATATTTCCTATTTAGT
TATTTAAAAAGCGTATCCATTTAGGGGGGGGGGGGAAACAAAAAAAAATTGAATCTTATA
ATTTCCAAAAATTTTTTGTCTAACAAAAAACCCCCCTTCTTGGGAAAGGCCCCAAAAAAG
GCGTTCATTTCCCCGGACGGGAAATTTCCAATTGAAAAGGGCTCCTTGCCCGGGAAATCT
CCTGGGATCACCAATGAGTATAAGGGCCAATTGGGAAAAAAAAAAAAATTATTTCCCATT
TTTTTTTCATTTCAAAAATGTAAATTCCCCC
   
Sequence Name 31-K113377-0022-086-D06C-8409
GenBank Accession LN500241 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K113377-0022-086-D06C-8409
GCATTTACATTGAATATATCCTGTCCGGGAACATGAACGCCTTATATGAGACATTACCAA
AGAAGGTAGATTATTTGTAAGACAAAAACTCTTAGTAACTTATAAGATTCAATTTTTTTT
TGTTACACACACACACATAAATGAATACGCTCTTCAAATAACTAAATAGAAAATATGTAG
TTAAATTGAACTAGTTAATTTTAATTAATCCAAATATTAAAAAGCAAGTAAATAGCCCGT
TATTCTTTTTTTTTGGAAATAAAAGGTTTATTCCTATTTTTTTTTTTTATGGGGCCAACC
AACTTTCTTTGGAAAAGGAAGGAAAAATTTAAAAAAAAATTTTTTTTTAAAAAAAGCAAA
AAAGGCCTTTTACATGGGGGTTACCCTTTTTCCCGCCCCAAA

Last Updated on 06.12.2018 16:11