Sequences from confirmation amplicon


Confirmation sequences for line 086E04 and hit At3g03540 are shown.
Line ID 086E04
Locus At3g03540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:848087
Primers used for PCR 24jx - 8474, show primer details
   
Sequence Name 29-K113531-0022-086-E04C-24jx
GenBank Accession LN499373 [GenBank]
Sequencing primer gene specific primer, 24jx: 5'-TTCGATCAGTAATTTCCTCTCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K113531-0022-086-E04C-24jx
ACATCGGTTTGGCTGTGTTGCACCCGGAACCGACGCAAACCACCTGTCCAATACATGAAA
TTTGCTATAAACCGGGATTTTGCTAGACAACCAATTTCCAATTCGGTTTTAGTTGGATGA
ACCGAACCGTTATACAGATGTAATTTATACAGGTCAAACGGTCTCACCTATCACATATAG
CGAAATTTTGAACCAATTCCTTGTAAACCGGCAAAGCGTCCGGTTTAAATCCGTTCATAA
CCGCAGAAGACATCCCTTTCATCTTTCGCTCGGCGTTTTGGGCAAATCCGGACATTGTAG
CTGGACCCGGGTTTGGGTCCGGGTGACCCGAATCCCATGGTTTACCGAACACTTGTTCGT
AAATATCCCGAATGGAGTGACCTGGATTCGGGTCAACGTAATTTCCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 04-K113530-0022-086-E04C-8409
GenBank Accession LN487995 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K113530-0022-086-E04C-8409
ATCCTGGAAATTACGTTGACCCGAATCCAGGTCACTCCATTCGGGATATTTACGAACAAG
TGTTCGGTAAACCATGGGATTCGGGTCACCCGGACCCAAACCCGGGTCCAGCTACAATGT
CCGGATTTGCCCAAAACGCCGAGCGAAAGATGAAAGGGATGTCTTCTGCGGTTATGAACG
GATTTAAACCGGACGCTTTGCCGGTTTACAAGGAATTGGTTCAAAATTTCGCTATATGTG
ATAGGTGAGACCGTTTGACCTGTATAAATTACATCTGTATAACGGTTCGGTTCATCCAAC
TAAAACCGAATTGGAAATTGGTTGTCTAGCAAAATCCCGGTTTATAGCAAATTTCATGTA
TTGGACAGGTGGTTTGCGTCGGTTCCGGGTGCAACACAGCCAAACCGATGGTTTATACAT
TCGGCAACATCACACGGAACAACGAACAACGAGNGGAAATACTGGATCG

Last Updated on 06.12.2018 16:11