Sequences from confirmation amplicon


Confirmation sequences for line 086E06 and hit At1g21890 are shown.
Line ID 086E06
Locus At1g21890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7683264
Primers used for PCR PA29 - 3144, show primer details
   
Sequence Name 29-K037017-022-086-E06C-3144
GenBank Accession LN499197 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K037017-022-086-E06C-3144
ATCCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGATCATAA
GCATACGAGCTTGTGGTGAAATGCTCCATGAAAGAGGTTTCGAGATAAGCAAGACTAAAG
AATCTTCGGTAACCACCACTCCTTGGAGGTCACTGTGTGCAATGCAGCTGCTAAAATTTT
ATCTGCTATTACTTAATTTTATAATCGCATATTACTTATAATTCTCTTTTACTTAATTTT
ATCTGCTAAAATTTTTGGACCCTATTACTTAATTTTATCTGCTAAAATTTTCAGTGTGAT
TGGAACGCTATTTATAATCGTGGGCCTATACACTGTTGTATGGGGTAAAGGCAAAGACAA
AAGAATGACGGATGATGACGAAGATTGCAAAGGACTTCCGATTAAAAGTCCGGTTAAACC
GGTAGATACCGGTAAAGGCTTAGCCGCAGAACTGGAGATGAAATCAAAGGAAGGTCAAGA
GGCAAAGGCTACTACTACTACTCAAGTTGAGATTTGAGGATGCTTATGTACTGTTGACGG
AGGAAGACACAAATCTGTCTTTCTTTCTCTGTATATTAAGTTTATGTTTTTTGCTTTCCA
AG
   
Sequence Name 30-K037017-022-086-E06C-PA29
GenBank Accession LN499627 [GenBank]
Sequencing primer gene specific primer, PA29: 5'-ATCTTTCTGTAACCTTGGAAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K037017-022-086-E06C-PA29
CAGAGAAGAAGAAGATTTGTGTCTTCCTCCGTCAACAGTACATAAGCATCCTCAAATCTC
AACTTGAGTAGTAGTAGTAGCCTTTGCCTCTTGACCTTCCTTTGATTTCATCTCCAGTTC
TGCGGCTAAGCCTTTACCGGTATCTACCGGTTTAACCGGACTTTTAATCGGAAGTCCTTT
GCAATCTTCGTCATCATCCGTCATTCTTTTGTCTTTGCCTTTACCCCATACAACAGTGTA
TAGGCCCACGATTATAAATAGCGTTCCAATCACACTGAAAATTTTAGCAGATAAAATTAA
GTAATAGGGTCCAAAAATTTTAGCAGATAAAATTAAGTAAAAGAGAATTATAAGTAATAT
GCGATTATAAAATTAAGTAATAGCAGATAAAATTTTAGCAGCTGCATTGCACACAGTGAC
CTCCAAGGAGTGGTGGTTACCGAAGATTCTTTAGTCTTGCTTATCTCGAAACCTCTTTCA
TGGAGCATTTCACCACAAGCTCGTATGCTTATGATCTGATAGTTTAAACCGAAGGCGGGA
AACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATA
GAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
TCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTC
CTCGTGGGTGG

Last Updated on 06.12.2018 16:11