Sequences from confirmation amplicon


Confirmation sequences for line 086G05 and hit At2g29380 are shown.
Line ID 086G05
Locus At2g29380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12609049
Primers used for PCR 28jx - 8474, show primer details
   
Sequence Name 62-K113393-0022-086-G05C-8409
GenBank Accession LN510805 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K113393-0022-086-G05C-8409
ATCCTCCCGAGAGCTTCGAGGAATTGAAGAGTATCTCGTAGCTAAAGCCTCCTGTTTACT
ACGCTTACAATTCTTCTCCCAATCTTCATGCATCACCGTTTGGAATCTCGGCCTCCGCCG
TGAATGTGCCGGCGTTGATTCATAAACCGACGACGGACATGCGTCGGTCACTACTTCGTA
ACATATCTCGGCCATATCCGGGGAAAAAAACCTTAAACTTTGATGTCTTCAAATGGATTT
TGCTCTGTATTTCTGATCAAGAAGAACAAAACTCTTTCTTAGATGTTTCAAGAAATGTTT
TCTTTTTTGATGAACTCTTTCAAGAACTGGTGATTCCTGTTTTGGTTGCTTCACTGGTTA
ATAAAAATGCTCGAAAACCATTCAATTTGAGAAAT
   
Sequence Name 81-K113394-0022-086-G05C-28jx
GenBank Accession LN514092 [GenBank]
Sequencing primer gene specific primer, 28jx: 5'-ATTTCTCAAATTGAATGGTTTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K113394-0022-086-G05C-28jx
CAGGAATCCCAGTTCTTGAAGAGTTCATCAAAAAAGAAAACATTTCTTGAAACATCTAAG
AAAGAGTTTTGTTCTTCTTGATCAGAAATACAGAGCAAAATCCATTTGAAGACATCAAAG
TTTAAGGTTTTTTTCCCCGGATATGGCCGAGATATGTTACGAAGTAGTGACCGACGCATG
TCCGTCGTCGGTTTATGAATCAACGCCGGCACATTCACGGCGGAGGCCGAGATTCCAAAC
GGTGATGCATGAAGATTGGGAGAAGAATTGTAAGCGTAGTAAACAGGAGGCTTTAGCTAC
GAGATACTCTTCAATTCCTCGAAGCTCTCGGGAGGATTTCTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11