Sequences from confirmation amplicon


Confirmation sequences for line 086H01 and hit At3g05020 are shown.
Line ID 086H01
Locus At3g05020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1391926
Primers used for PCR 04is - 8474, show primer details
   
Sequence Name 43-K112877-0022-086-H01C-8409
GenBank Accession LN504897 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K112877-0022-086-H01C-8409
TCATTGAATATTCCTGTGCTTTCTCTTCGGCCATTTGGATGTTAAACTCTTCCTCTAAAC
CCATTACTATCTCAACCTGAAGAAAAACACTCAGTTGATCAGTATCAAGAAAATTAAGAA
TTTGTGTTAATTGCCACTTGAGTGAATGGATACCGTGTCGAGAGAATCTGCTCCAAGGTC
AGCAAATTTGGTTTCTGCAACGACTTTTTTATCCGGTGTAAGTGATAGTTGCTTCTTAAC
TATAGCAGACACTTTCTCTATCGTCTCTTGTTTTGCCTATAAACCAAATATAATGTCTTT
TAAAACTTTGAGCCCTATATATATAGATTAACAAACTTGAAAAAAAATGCTCATACCGCG
CAAGAAACAGAGAGGCGGCGAGATGGGATTGAACGGCGGAGGTTGAAGGATAGATTAGTC
TTTCCATGGTTGGAAATCAAAGCTGGCTTTTGGAAACTAATCCTTGTTGTTGCCTATTTG
TTTCATCAAACACACACAAAAAAAAAAAGCTTTGCATTCATAAAAACTTTGTTCTAAATT
TTCATAACTCAGCCCAAATTCTAACCCAATGAAGGTCCTTGCACATTTTTCCACGCCCAA
AATTA
   
Sequence Name 80-K112878-0022-086-H01C-04is
GenBank Accession LN513966 [GenBank]
Sequencing primer gene specific primer, 04is: 5'-TGTGGAAGTGAATGCTTACTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K112878-0022-086-H01C-04is
GCTAGAATTTGGCTTGAGTATGAAAATTCAGAACAAAGTTCTTATGAATGCAATGGCTTT
TTTTTTTGGGGGGGGTTGGAGGAAACAAATGGGCACCACCAGGGATTATTTCCCAAAACC
CACCTTTGATTCCCACCCAGGGAAAAACAAATCTATCCTTCAACCTCCGCCGTTAAATCC
CATCTCGCCCCCTCTTTTTTTCTGGCGCGGAAGGAGCATTTTTTTCCAAGTTGGTAAACC
TATATATATGGGGCTCAAAGTTTAAAAAAACTTTATATTGGGTTTATGGGCAAAACAAAA
AACAATGAAAAAGGTGTCGGCTATATTTAAAAACCACCTATCATTTACCCCGAAAAAAAA
ATTCTTTGCAAAACCCAAATTTGCTAACCTGGAACCAAATCCTCTCAACCCGGTTCCCTT
TCACTCAGGGGGCAATAAACACAAATCCTAAATTTTCTGGATCCGGATAACCGGAGGGTT
TTCCTCCGGGTGAAAATAGAAAGGGGTTAAAAGAAAAATTTTAACACCCAAATGGCCAAA
AAAAAACCACGGAAAATTTCCATTTGAAAAGGGCTCCAGGCCCGGAAAATCTCCTGGGAT
CACCATTGAGTATAAGGGTCAATATGGAAAAAAAAAAAAAGTATTTCCCATTTTTTTTCC
ATTTCAAAAATGAAAATTCCCCC

Last Updated on 06.12.2018 16:11