Sequences from confirmation amplicon


Confirmation sequences for line 087C02 and hit At4g26650 are shown.
Line ID 087C02
Locus At4g26650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13446105
Primers used for PCR 10ra - 8474, show primer details
   
Sequence Name 81-K115912-0022-087-C02C-10ra
GenBank Accession LN514108 [GenBank]
Sequencing primer gene specific primer, 10ra: 5'-CTAGACGGTAAACCTCCAACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K115912-0022-087-C02C-10ra
TTTTAGCACTTGCTGATTATCTCGCGGGACAGCTTTTCTTTGCCTCGACCTGCAACAAAG
AAATAAAAAAAGATCTTTCATTTCTTTTTCTCATTGATCTAACACTAACATTGCCACTTT
CAACACAAAGCAACAACACATTCTCACAAGTTATTACCATTAACAGCAAATCCAGAAGCT
AATGACAACAGAAGATTTCGAAAAGAGCATGATTACAGAAAGATCTAAACGTTGCTGCTT
AAAACTCATTAATGGATGACACCAAACAGATCTCTAAGAGAGCATGAATACAGAAAGATC
GAGATTATAGAACAATCCGTTGCTCATTAACGAGCAGCGAGACCAATAAGACCTCTAAGA
CAGAATCATTACAGAAAGATCGAGACTTTAGAACACTCGATTACTCAAGACTGATAATTA
GATGAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTCATTCAAAAATGTA
   
Sequence Name 64-K115911-0022-087-C02C-8409
GenBank Accession LN511270 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K115911-0022-087-C02C-8409
TCGAGCAACGGAATTCGTTCCTATAATCTCGATCCTTTTCTGTATTTCATCGCTCTCTTA
GAGATCTGTTTCGGTGTCATCCATTAATGAGTTTTAAGCAGCAACGTTTAGATCTTTCTG
TAATCATGCTCTTTTCGAAATCTTCTGTTGTCATTAGCTTCTGGATTTGCTGTTACTGTT
ATAACTTGTGAGAATGTGTTGTTGCTTTGTGTTGAAGTGGCAATGTTAGTGTTAGATCAA
TGAGAAAAGAATGAAAGATCTTTTTTTATTTCTTTGTTGCAGGTCGAGGCGAAGAAAGCT
GTCCCGCGGGATGATCAGCAAGTGCTAAAACGACACGCCAGTCCAATGCACCTTATCTCA
CCTAGCCATGGTGGTAATGGTGGTGGAGCACGGACAAAGAAGATCTTTGTTGG

Last Updated on 06.12.2018 16:11