Sequences from confirmation amplicon


Confirmation sequences for line 087F12 and hit At1g20960 are shown.
Line ID 087F12
Locus At1g20960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7309132
Primers used for PCR 46uj - 8474, show primer details
   
Sequence Name 50-K117452-0022-087-F12C-46uj
GenBank Accession LN507299 [GenBank]
Sequencing primer gene specific primer, 46uj: 5'-TAGAAACCCTGAGAAAAAGATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K117452-0022-087-F12C-46uj
GAAGTTAATCCACGGACTTCCCAGAGGGTGGTGATTCTGGTGGTGGTAGAGCTAATGATG
ATGAAGGGCTTGATGATGATTTGGGCGTGGCTGTTGAATTTGAGGAGAATGAAGAAGATG
ATGAGGAAAGCGATCCTGATATGGTTGAAGAGGATGATGATGAGGAGGATGATGAGCCTA
CCAGAACTGGAGGCATGCAGGTTGATGCAGGGATAAATGATGAGGATGCAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 27-K117451-0022-087-F12C-8409
GenBank Accession LN498575 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117451-0022-087-F12C-8409
ATCCTGCATCCTCATCATTTATCCCTGCATCAACCTGCATGCCTCCAGTTCTGGTAGGCT
CATCATCCTCCTCATCATCATCCTCTTCAACCATATCAGGATCGCTTTCCTCATCATCTT
CTTCATTCTCCTCAAATTCAACAGCCACGCCCAAATCATCATCAAGCCCTTCATCATCAT
TAGCTCTACCACCACCAGAATCACCACCCTCTTGGAAGTCCGTGATTAACTTTCCAATGG
AAACAAGCTGATCAAACTCATGGTTTTCAATCTTGTTCAGCAACTTCTCAATCTCCATCT
TTTTCTCAANGGGTTTCTA

Last Updated on 06.12.2018 16:11