Sequences from confirmation amplicon


Confirmation sequences for line 087H01 and hit At4g20240 are shown.
Line ID 087H01
Locus At4g20240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10932154
Primers used for PCR 06qv - 8474, show primer details
   
Sequence Name 59-K115779-0022-087-H01C-06qv
GenBank Accession LN510077 [GenBank]
Sequencing primer gene specific primer, 06qv: 5'-CAGGCTTATCATCTCCTACTGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K115779-0022-087-H01C-06qv
GTTCCAGGTTGGCTAGTCCAATTTGGACAAACCAAAGAAAATCCGATTCCAGGGCAAAGT
CTTCTCCCTGCTCCAAATGGAACGAATTTGGAATCAGTACGTTGAAATCCAAATTCGTAC
CAAAATGTCTCTCCGGTCTATATTCATTGGCATCAAGCCCCCATATCGCAGGGTTTCGTT
GGAGTGCCCAAGCATTGATTATGACCTGTGTTCCAAGTCGAGCACATTTTTACATTTAGG
TTTACTCTTATAAGAATTTTAAAAAAGAAAGAAAAAAAAAACGTTTACATGTGTCCCTGC
AAAAATGTCATATCCCTTTAATTGGACATCTTCACTTGGTAGTCTGAACAATAGTGGACC
AAAGGGATGCAACCGTAGCCCCTCCTTAATCACGCAATGTAAATAGTTCATTTTCTCAAC
ATGAATTAATCTACATGGCTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAAATA
   
Sequence Name 11-K115778-0022-087-H01C-8409
GenBank Accession LN491263 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115778-0022-087-H01C-8409
TCATTGAGCCTGTAGATAATTCATGTTGAGAAAATGAACTATTTACATTGCGTGATTAAG
GAGGGGCTACGGTTGCATCCCTCTGGTCCACTATTGTTCAGACTACCAAGTGAAGATGTC
CAATTAAAGGGATATGACATTTCTGCAGGGACACATGTAAACGTTTTTTTTTTCTTTCTT
TTTTAAAATTCTTATAAAAGTAAACCTAAATGTAAAAATGTGCTCGACTTGGAACACAGG
TCATAATCAATGCTTGGGCACTCCAACAAAACCCTGCAATATGGGGGCTTGATGCCAATG
AATATAGACCGGAAAGACATTTTGGTACAAATTTGGATTTCAACGTACTGATTCCAAATT
CGTTCCATTTGGAGCAGGGAGAAAACTTTGCCCTGGAATCGGATTTTCTTTGGTTTTGTC
CAAATTGGCACTAGCCAACCTTGTGAAACGATTCAACTGGAGACTCAGGGTCGGACCATT
AGGAGATGATAAGCCGG

Last Updated on 06.12.2018 16:11