Sequences from confirmation amplicon


Confirmation sequences for line 088E02 and hit At1g48860 are shown.
Line ID 088E02
Locus At1g48860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18069486
Primers used for PCR 04wu - 8474, show primer details
   
Sequence Name 57-K119004-0022-088-E02C-04wu
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 04wu: 5'-GACACAAATACCGCAAAATTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K119004-0022-088-E02C-04wu
AGAATTTTACTTTTCTAAGCTCTGTGCAAATGGCAATCATCCTTTCCGTCTCCTTTACTC
TCCAGCTAGCCACTATATTGACCAAATAGAATTAAGTGACCATGAATCTTGAGAAACCTT
ATATTATAAGAGAGAGCTTTTTACTTACCATCTCTAATGGTGGTTGGACCATCGGCAAAG
AGAGCAACGACGGCAAGAGTCATTGCTACATCAGGCATTTTGTTCATGTTGACATCAATA
GCCCGCAAGTGTCTCATTCCAAAAGCATCTCTAGACGGCCCTGTCACAGTCACACTGTTC
TCTGTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATG
   
Sequence Name 40-K119003-0022-088-E02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K119003-0022-088-E02C-8409
CATTTCATTGAATTATCCTGGACAGAGAACAGTGTGACTGTGACAGGGCCGTCTAGAGAT
GCTTTTGGAATGAGACACTTGCGGGCTATTGATGTCAACATGAACAAAATGCCTGATGTA
GCAATGACTCTTGCCGTCGTTGCTCTCTTTGCCGATGGTCCAACCACCATTAGAGATGGT
AAGTAAAAAGCTCTCTCTTATAATATAAGGTTTCTCAAGATTCATGGTCACTTAATTCTA
TTTGGTCAATATAGTGGCTAGCTGGAGAGTAAAGGAGACGGAAAGGATGATTGCCATTTG
CACAGAGCTTAGAAAAGTAAAATTCTTCTTCTCTCTCTCTCTTTCTGTTTACAGTGCTCA
TTCTAAGAAATTTTGCGGTATTTGTGT

Last Updated on 06.12.2018 16:11