Sequences from confirmation amplicon


Confirmation sequences for line 088E04 and hit At5g36950 are shown.
Line ID 088E04
Locus At5g36950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14595432
Primers used for PCR 05pv - 8474, show primer details
   
Sequence Name 25-K115371-0022-088-E04C-05pv
GenBank Accession LN497584 [GenBank]
Sequencing primer gene specific primer, 05pv: 5'-AAATTGCTCTTCTTTCATGCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K115371-0022-088-E04C-05pv
GCTATATGTAGCAGAGCTACTTCATTACCTTGAGGATAGCCAACTACAGCCACAGCTTCC
TGAAGAAACGGTATATCTCCTAGCTCCAAAGCATTCATACCTTCCCAAAACACTTCACTA
TCAACTACCAATATAGCCAAATCACATTCATGACCAACCGCTTGAACTTCTGCTCTGTGT
TTTATCGACGATCCATGCTTTCTAACTAACACAAAGGAGTGATCAGCCACGACATGCGCA
TTCGTTATAATCTTCCTTCCAGAAATCACAAATCCTAAATCAACACCAAACAGAAAATTC
AATCTAAAAATCACCAAAATCACACTTGAAGCAATTCCAAAGAAAGAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 05-K115370-0022-088-E04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115370-0022-088-E04C-8409
TCGCTGCCCTCGCCCGGGTCGGTTGTCTCTTCTTG

Last Updated on 06.12.2018 16:11