Sequences from confirmation amplicon


Confirmation sequences for line 088E06 and hit At1g48490 are shown.
Line ID 088E06
Locus At1g48490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17923903
Primers used for PCR 09sm - 8474, show primer details
   
Sequence Name 39-K116453-0022-088-E06C-09sm
GenBank Accession LN503484 [GenBank]
Sequencing primer gene specific primer, 09sm: 5'-GGGTTGCTTATTGATGTTTACGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116453-0022-088-E06C-09sm
GACTATTTTACTACCATATGACTGGACAGAAAATCACTTACCGATCTATCAAATCACGGG
CTTCATGGCTCATATCTTCAGGAACAGGAGGCCATTGTATATTACGGTTGAGAATATTGT
CAAAGATTTGCTAAAACCCAACAAAGAAGATACAGATCAAATATCAGGGAGTTGGAGAGT
GAAACTAGATATTAAATACTCTGTAGCAACATATGAAGATGTTAAATGGAAGTGTTTGAA
AGTTAAGAGGCCAGTACAAAGAAACATAGCATTTGGCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTATAAT
   
Sequence Name 06-K116452-0022-088-E06C-8409
GenBank Accession LN488971 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K116452-0022-088-E06C-8409
ATTACATTGCCAAATGCTATGTTTCTTTGTACTGGCCTCTTAACTTTCAAACACTTCCAT
TTAACATCTTCATATGTTGCTACAGAGTATTTAATATCTAGTTTCACTCTCCAACTCCCT
GATATTTGATCTGTATCTTCTTTGTTGGGTTTTAGCAAATCTTTGACAATATTCTCAACC
GTAATATACAATGGCCTCCTGTTCCTGAAGATATGAGCCATGAAGCCCGTGATTTGATAG
ATCGGTAAGTGATTTTTCTGTCCAGTCATATGTGTAGTTAAAATAGTTCTTTATGAATTT
GTCAGCAGCTCTATCGTAAACATCATACAGCAACCCA

Last Updated on 06.12.2018 16:11