Sequences from confirmation amplicon


Confirmation sequences for line 089B08 and hit At4g23670 are shown.
Line ID 089B08
Locus At4g23670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12333554
Primers used for PCR 11rn - 8474, show primer details
   
Sequence Name 33-K116321-0022-089-B08C-11rn
GenBank Accession LN501140 [GenBank]
Sequencing primer gene specific primer, 11rn: 5'-CCGTGATTATCTTCTAAAGGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116321-0022-089-B08C-11rn
CATTAAAATAACAATTTAATTGAAAGCTCCTTCAACAAAAAAGTTAAAAAACAACACACA
TCTATCATTAAATAGTGTTTGAGTTAAAAAAGAAACAGAGAAGATTTTAGGTATGCACTA
TCAAATGATTTAAAGAAACGGTAGAAACCTTGTTTTTAACCGTCACGGTCCCTATATAAA
CCTATGAGCTAGCGACGACAAGTATTCAGTATAATCAATCAGAAAACAAGTAGAAACTTT
AAAACGAGAGAGAGAGAAAGAAATGGCGACATCGGGAACGTACGTGACGGAGGTTCCGTT
GAAAGGATCGGCGGAGAAACACTACAAGAGCTGGAAGAGCGAGAACCATGTCTTCGCTGA
CGCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTC
   
Sequence Name 01-K116320-0022-089-B08C-8409
GenBank Accession LN486728 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116320-0022-089-B08C-8409
TACATTGGCGTCAGCGAAGACATGGTTCTCGCTCTTCAGCTCTTGTAGTGTTTCTCCGCC
GATCCTTTCAACGGAACCTCCGTCACGTACGTTCCCGATGTCGCCATTTCTTTCTCTCTC
TCTCGTTTTAAAGTTTCTACTTGTTTTCTGATTGATTATACTGAATACTTGTCGTCGCTA
GCTCATAGGTTTATATAGGGACCGTGACGGTTAAAAACAAGGTTTCTACCGTTTCTTTAA
ATCATTTGATAGTGCATACCTAAAATCTTCTCTGTTTCTTTTTTAACTCAAACACTATTT
AATGATAGATGTGTGTTGTTTTTTAACTTTTTTGTTGAAGAGCTTTCAATTAAATTGTTA
TATTTAATGTTGGACCACGTTATTTACGTGGGTAGTTGGCACAGCATCCTTTA

Last Updated on 06.12.2018 16:11