Sequences from confirmation amplicon


Confirmation sequences for line 089B12 and hit At1g80260 are shown.
Line ID 089B12
Locus At1g80260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:30179479
Primers used for PCR 39vh - 8474, show primer details
   
Sequence Name 43-K117914-0022-089-B12C-39vh
GenBank Accession LN504989 [GenBank]
Sequencing primer gene specific primer, 39vh: 5'-TTTTCATCTTACTCAAGCCATGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K117914-0022-089-B12C-39vh
GCCACTCTACCAACTTATATTACTCCACGAAAAATGATACTGTAATTATAACCAATCATT
TAGCATTGGACAATACCCTGTCCATTACGTATTGATGAAAAGCATCCACAAAGTTGAGGA
GCTTTTGTTCTAGTAACCAGTGGTGCTTGCGGATTTTTGTTGCAGAGCCTTTACCCTATC
ATACACAAACACCAAAGTCATCAAGTTTATCAAACTGAACCACATTCTTATGATTCCCTT
TATATTTATATGTATAGATCAAAAGACTGATTAGGCTCTTTCAAACTGGATTTGGAAGTT
CATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGC
   
Sequence Name 03-K117913-0022-089-B12C-8409
GenBank Accession LN487669 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117913-0022-089-B12C-8409
ATTGATGAACTTCCAATCCAGTTTGAAAGAGCCTAATCAGTCTTTTGATCTATACATATA
AATATAAAGGGAATCATAAGAATGTGGTTCAGTTTGATAAACTTGATGACTTTGGTGTTT
GTGTATGATAGGGTAAAGGCTCTGCAACAAAAATCCTCAAGCACCACTGGTTACTAGAAC
AAAAGCTCCTCTACTTTGTGGATGCTTTTCATCAATACGTAATGGACAGGGTATTGTCCA
ATGCTAAATGATTGGTTATAATTACAGTATCATTTTTCGTTGGAGTAATATAAGTTTGTG
CTAGAGTTGTGGCAAAATCAAAGGCTGATCACATGGCTTGAGTAAGATGAAAAATAANAA
AAGGGTGTTTCCCATTTTTTTTTTATTCAAAAAA

Last Updated on 06.12.2018 16:11