Genotyping and primer details


Genotyping and primer details for 089B12 - At1g80260
Line ID 089B12
Locus At1g80260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30179479 of line 089B12 Chr1:30179479
   
Insertion allele amplicon 8474 - 39vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 39vh: 5'-TTTTCATCTTACTCAAGCCATGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30179824
   
wt amplicon (over InsPos) 33vu - 39vh
wt amplicon start Chr1:30178764
wt amplicon end Chr1:30179824
wt amplicon size 1061 bp
Locus specific primer 39vh: 5'-TTTTCATCTTACTCAAGCCATGTG-3'
2nd locus specific primer 33vu: 5'-CAGGTAACCAGGTTTGGTGTATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11