Sequences from confirmation amplicon


Confirmation sequences for line 089D11 and hit At1g58390 are shown.
Line ID 089D11
Locus At1g58390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21693740
Primers used for PCR BH81 - 8474, show primer details
   
Sequence Name 05-K104381-0022-089-D11C-BH81
GenBank Accession LN488339 [GenBank]
Sequencing primer gene specific primer, BH81: 5'-ACAAAATCGCTTTCGTAGTCCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K104381-0022-089-D11C-BH81
CCTTGAGGTTGATATAACCACCATCAACGATAGCTTGTTGCACTCCAAAACTCTGCATAT
CTCGGATCACATCCGAGATCCTCGTACGTATGCCTCCAACATCTAATGCATTTCTCCTAC
GATCTGAAATAATGCAAGCATGTCTTCTGATACGCATCTTGATTCCACTTGTTTTCCAAA
GTTTTTCTTTAAGAAGATATGTTTCAATTATATCTTCTGCGTCGTAAACAATTTCCTTTA
TTTCTTCGACAACATTTCTCACCACCGCAGTTGTATGTTTCTAGCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCAC
GGTTATA
   
Sequence Name 21-K104382-0022-089-D11C-8409
GenBank Accession LN495645 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104382-0022-089-D11C-8409
GCCTTTACATTGAATATATCCTGCTAGAAACATACAACTGCGGTGGTGAGAAATGTTGTC
GAAGAAATAAAGGAAATTGTTTACGACGCAGAAGATATAATTGAAACATATCTTCTTAAA
GAAAAACTTTGGAAAACAAGTGGAATCAAGATGCGTATCAAAAGACATGCTTGCATTATT
TCAGATCGTAGGAGAAATGCATTAGATGTTGGAGGCATACGTACGAGGATCTCGGATGTG
ATCCGAGATATGCAGAGTTTTGGAGTGCAACAAGCTATCGTTGATGGTGGTTATATGCAA
CCTCAAGGTGATAGACAAAGGGAGATGCGACAAACGTTTTCTAAGGACTACGAAGCGNAT
TTT

Last Updated on 06.12.2018 16:11