Sequences from confirmation amplicon


Confirmation sequences for line 090C01 and hit At5g54200 are shown.
Line ID 090C01
Locus At5g54200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21994296
Primers used for PCR 14eq - 8474, show primer details
   
Sequence Name 48-K110825-0022-090-C01C-14eq
GenBank Accession LN506530 [GenBank]
Sequencing primer gene specific primer, 14eq: 5'-GTTTCTCCATCTCGTCTTTTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K110825-0022-090-C01C-14eq
TACAACTGCAGCAGAGACGCGAAATCAATCTGAAGAACAGGAAAAAGAAAACCCATCACA
AGAAAATCACTGGGTTTTCAGGTAAAGCTAAAACAACTTACACCGTTCTGTTCTCATCTT
TTCATTTATACTACAATTTTGTTTTGGATGCTGTACTGGTTTATCTGACTATTCTCGTGA
AACTTTCACAGTTTGTGGCAGGAAGTTCATCGGAAGTTCTCGTCACATCTGCAGATTCAC
GTACGCGTGTTGTTGATGGTGTTGACCTTGTTCACAAGTTTAAAGGTAAACAATACTAAG
CTTAAAACTCTCTTCTCCATTCTTAGAACTCGGGAGTTAGGAATAACTTTATTGTTCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCACACGNTTATTA
   
Sequence Name 32-K110824-0022-090-C01C-8409
GenBank Accession LN500630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K110824-0022-090-C01C-8409
CGGACTGAGCCTTTACATTGAATATATCCTGAACATAAAGTTATTCCTAACTCCCGAGTT
CTAAGAATGGAGAAGAGAGTTTTAAGCTTAGTATTGTTTACCTTTAAACTTGTGAACAAG
GTCAACACCATCAACAACACGCGTACGTGAATCTGCAGATGTGACGAGAACTTCCGATGA
ACTTCCTGCCACAAACTGTGAAAGTTTCACGAGAATAGTCAGATAAACCAGTACAGCATC
CAAAACAAAATTGTAGTATAAATGAAAAGATGAGAACAGAACGGTGTAAGTTGTTTTAGC
TTTACCTGAAAACCAGTGATTTTCTTGTGATGGGTTTTCTTTTTCCTGTTCTTCAGATTG
ATTTCGCGTCTCTGCTGCAGTTTGTTATCTGAATAAACAAAAAAGATAATAAGTTCAAAA
GACGAGAGNGAGAAA

Last Updated on 06.12.2018 16:11