Sequences from confirmation amplicon


Confirmation sequences for line 090C02 and hit At3g21770 are shown.
Line ID 090C02
Locus At3g21770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7674726
Primers used for PCR IH36 - 8474, show primer details
   
Sequence Name 72-K100854-0022-090-C02C-8409
GenBank Accession LN512682 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K100854-0022-090-C02C-8409
GCCATTTACAATTGAATATATCCTGCATTATAGGCTTGTCTTGAAGAGGAGAGGCTTGTT
TCAGTCTGATTCTGCATTAACAACGAACTCAGCGACTTTGAAGGTGATCAACGACTTGGT
CAACGGTTCTGAAAAGAAGTTTTTCAAAGCTTTTGCTAAGTCAATGGAGAAGATGGGGAG
AGTTAAAGTGAAGACTGGCTCAGCTGGTGTGATCAGGACACGGTGTTCTGTCGCTGGAAG
TTAGTAAGCTTCAGGGTCGGAATGTGGTGTGTTATGGTCATTTAATTTACAAATGTTGTT
AATTTAATTTTGTTGTTGTTTGATTTGTGTTTATGCACCCAAGAATGATGTGTTATGGTT
CAATTAATATGTGAATCATTTAAATTTACAATAATTCTTATTATTCTGTATCTAGCCTTC
TAATCCAAGGTAAATGAGTTTGAAACTGTAAGAAACTTTCGTGTCTCCTTGAATCCGGCA
TAGATCTTTTCATTATTTTCATACATTCACTAAGTTCATACACAAAACTTAAAAATACAT
GTTCACAGAAATAAAACAGCTTGACAATGGCCCATTATTCAGGCCCATTATACAACATTT
TCAATCGA
   
Sequence Name 72-K100860-0022-090-C02C-IH36
GenBank Accession LN512683 [GenBank]
Sequencing primer gene specific primer, IH36: 5'-AAGCTAGGGAAATCGATTGAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K100860-0022-090-C02C-IH36
CAGCTGTTTATTTCTGTGAACTGTATTTTTAAGTTTTGTGTATGAACTTAGTGAATGTAT
GAAAATAATGAAAAGATCTATGCCGGATTCAAGGAGACACGAAAGTTTCTTACAGTTTCA
AACTCATTTACCTTGGATTAGAAGGCTAGATACAGAATAATAAGAATTATTGTAAATTTA
AATGATTCACATATTAATTGAACCATAACACATCATTCTTGGGTGCATAAACACAAATCA
AACAACAACAAAATTAAATTAACAACATTTGTAAATTAAATGACCATAACACACCACATT
CCGACCCTGAAGCTTACTAACTTCCAGCGACAGAACACCGTGTCCTGATCACACCAGCTG
AGCCAGTCTTCACTTTAACTCTCCCCATCTTCTCCATTGACTTAGCAAAAGCTTTGAAAA
ACTTCTTTTCAGAACCGTTGACCAAGTCGTTGATCACCTTCAAAGTCGCTGAGTTCGTTG
TTAATGCAGAATCAGACTGAAACAAGCCTCTCCTCTTCAAGACAAGCCTATAATGCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCG

Last Updated on 06.12.2018 16:11