Sequences from confirmation amplicon


Confirmation sequences for line 090F11 and hit At1g76530 are shown.
Line ID 090F11
Locus At1g76530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28719768
Primers used for PCR NR06 - 8474, show primer details
   
Sequence Name 50-K036418-022-090-F11C-8409
GenBank Accession LN507088 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K036418-022-090-F11C-8409
GAAGCCTTTCAATTGAATATATCTTTGAAAAACTCACTTTTTCTGTGTCTTGTTTCTTTT
GGCTAAAGAGATGGGGCCATTCCTGCTATGACCCTGATTCTTGGAGGAAACTTACTTAAA
GGAATGAGGAGGTCGGAAGTGAGGAGTTCAGAAATGAAAAACTCCTGCATTATCGGCGTC
TTGGTAGCTCGTTACATTTTGCTACCTGTAAGCGGTGTTTTACTCGTGAGAGGAGCATAC
AAGTTGGATTTAGTAACCTCAGAACCCTTGTACCAATTCGTTCTTCTTCTTCAGTATGCT
GTCCCACCAGCCATGAATCTAGGTGCATGGCTTGAAACCAACTCCTTAAAATACAGTTAC
TTCTTTCAGATAAGATACATCACTAGTTTCCCTTACTTTTCAGGTACAAAGACCCAATTA
TTCGGCGCTGGGGAGAGTGAATGCTCTGTGATTATGCTTTGGACTTACTCTTTGGCTGCA
GTTTCACTCACTGTTTGGCCAACATTCTTCATGTGGCTTGTGACATAAATCCCCACACTC
GATCTTGTTATATGTATCCATTTATTCAAGAACTTATTCTTAGACTTCCAGATGACTGGA
   
Sequence Name 58-K036418-022-090-F11C-NR06
GenBank Accession LN509633 [GenBank]
Sequencing primer gene specific primer, NR06: 5'-TGATCCAGTCATCTGGAAGTCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K036418-022-090-F11C-NR06
ATGGATACATATAACAAGATCGAGTGTGGGGATTTATGTCACAAGCCACATGAAGAATGT
TGGCCAAACAGTGAGTGAAACTGCAGCCAAAGAGTAAGTCCAAAGCATAATCACAGAGCA
TTCACTCTCCCCAGCGCCGAATAATTGGGTCTTTGTACCTGAAAAGTAAGGGAAACTAGT
GATGTATCTTATCTGAAAGAAGTAACTGTATTTTAAGGAGTTGGTTTCAAGCCATGCACC
TAGATTCATGGCTGGTGGGACAGCATACTGAAGAAGAAGAACGAATTGGTACAAGGGTTC
TGAGGTTACTAAATCCAACTTGTATGCTCCTCTCACGAGTAAAACACCGCTTACAGGTAG
CAAAATGTAACGAGCTACCAAGACGCCGATAATGCAAGAGTTTTTCATTTCTGAACTCCT
CACTTCCGACCTCCTCATTCCTTTAAGTAAGTTTCCTCCTAGAATCAGGGTCATACCATG
AATGGCCCCATCTCTTTAGCCAAAAGAAACAAGACACAGAAAAAGTGAGTTTTTCAAAGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACTAATT

Last Updated on 06.12.2018 16:11