Sequences from confirmation amplicon


Confirmation sequences for line 090F12 and hit At2g39855 are shown.
Line ID 090F12
Locus At2g39855 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16637870
Primers used for PCR BH79 - 8474, show primer details
   
Sequence Name 07-K104381-0022-090-F12C-BH79
GenBank Accession LN489255 [GenBank]
Sequencing primer gene specific primer, BH79: 5'-AACATTTTAAGGACAAACACGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K104381-0022-090-F12C-BH79
AACTCACTTGGAGTCTCAAAATTTGTAGGTTGATACTTGGCAGCTCCATTTCGACTTGTC
AAGTTCCCTTGCGTCACCTTGAGTTGAGAACAAAGAAAAATTTCGTGAAACTGATGTATA
TCTTTTAGAATGATGATGATCATGATATATATTGGTTCATATATACCGCTGAAGGTGGTG
GGAAGATGGATTTGAACAATCCTGTTGTTGATGAAGAATACGAGTGAGAGACTCTTGGAC
CAAAAATATGATCCAACGACGAAGAGGACGATGAAGAGGAACATCGCTTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 23-K104382-0022-090-F12C-8409
GenBank Accession LN496541 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104382-0022-090-F12C-8409
GCGATGTTCCTCTTCATCGTCCTCTTCGTCGTTGGATCATATTTTTGGTCCAAGAGTCTC
TCACTCGTATTCTTCATCAACAACAGGATTGTTCAAATCCATCTTCCCACCACCTTCAGC
GGTATATATGAACCAATATATATCATGATCATCATCATTCTAAAAGATATACATCAGTTT
CACGAAATTTTTCTTTGTTCTCAACTCAAGGTGACGCAAGGGAACTTGACAAGTCGAAAT
GGAGCTGCCAAGTATCAACCTACAAATTTTGAGACTCCAAGTGAGTTTACTGCTTACTCT
AGTGAAGAGTTTTGGACGTGTTTGTCCTTAAAATG

Last Updated on 06.12.2018 16:11