Sequences from confirmation amplicon


Confirmation sequences for line 091A04 and hit At5g54870 are shown.
Line ID 091A04
Locus At5g54870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22289823
Primers used for PCR DR71 - 8474, show primer details
   
Sequence Name 53-K035533-022-091-A04C-DR71
GenBank Accession LN508073 [GenBank]
Sequencing primer gene specific primer, DR71: 5'-TGATTCTAATTGTATCAACCGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K035533-022-091-A04C-DR71
CGATTTGCTTCCGCATTTGCCCAGACAAGTCTCATGGCCGATTCTCAACTCTCTTTACGG
CGCCGCCGATCTGCTTCCTACTTTCATCGGAACAGCGAGTTCTGGGAACGATTCCGTTGA
CTGGAAAGGTGCTTGTTTCTTTGAGAACACCGCTTGGTTAGAGTTTCATAACAAGTCTGG
TAGCGAGTTTGGTGGAGGCACTCTTCACATTAAGGTGAAATTCAAAATTTGTAGTCGAAA
TCAATTTTGTTCCTTAGATCGTTCTGTTGCTTATTGAGTGATTTATGGGAGGTTTTTGTA
TATGGGTCCTCTTCAATTTTGACTTTTGAAACTTCTATTGTTGACCGTGAAGTCTAGAAG
TTTTGGGATCCTGTAATTTAGGAGAAGATTTCGAATTGTATTTGATCTTCTTCTCTAGTG
GATACTCTTAGAAGATGATGCTTCTTAATTCTTACACAAAGTTTTGGATTTTTTTTTTTT
TTTTTGTATTCCAATTTTGATTATTTTGTTTAAAAAGGCGGAAAAGGCCCATATTTGGAC
TTGTATGGATCTTTATTTTTTTCCAAAACAAATTTAACCCGGGATATTTTCAATTGTAAA
TGGCTTCCTGTCCGGAAAATCTACTGGGACCACAAATGATTAAGAGGGTCAATAAAGGAA
AAAAAAAAAAAATAATCACCATTTTTTTTTTCTTTCAAAA
   
Sequence Name 05-K035533-022-091-A04C-8409
GenBank Accession LN488260 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K035533-022-091-A04C-8409
TGAATATATCCTGGATCAGCTATGTATTGCAAATACCTAAAGATCCCTACATGTCCCACT
ATGTGCCTTATCCGCCTTCATAAACAACATAATCAAACTTAGAATACAAAAAAAAAAAAA
AAAGGCCCAAATTTTGGGTAGAAATTAGAAACCGCCCCCTTCAAAAAGTATCCCCGAAAG
AAAAAAATAAAAAACCATTCAAAACCTCCCCCAAAATTAGGGGACCCCAAACTTTCGAGA
CTTCCGGGCCAACAAAAAAATTTTCAAAATGCAAATTGGAGAAGGACCCGTATACAAAAC
CCCCCCAAAAATCCTCCGATAAGCCACAAAACGATTTAGGGAAAAAATTTGATTTCAACC
ACAATTTTGGAATTTCCCCTTAGGGGGAAGAGGGCCCCCCCCAAACCCCCTCCCAAATTT
GTTAGGAACCTCTACCCAGGGGGGGTTCCCAAAAAAACAAGCCCCTTTCCGGTCAAGGGA
ATCTTTCCCAAAACTCCTTTTTCCAATAAAATTGGGAACCAAATGGGCGGCCCCCAAAAA
AGAGTTAAAAATCGGCCATGAAACTTGTCGGGGCAAAGGCGGAACCAAATCCGGGGGATA
AAACGGGAATTTCCCGGATCCGCCGGTGGAAACAATTAAAAAAA

Last Updated on 06.12.2018 16:11