Sequences from confirmation amplicon


Confirmation sequences for line 092A07 and hit At5g58480 are shown.
Line ID 092A07
Locus At5g58480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23642442
Primers used for PCR 09ew - 8474, show primer details
   
Sequence Name 27-K110985-0022-092-A07C-09ew
GenBank Accession LN498444 [GenBank]
Sequencing primer gene specific primer, 09ew: 5'-CAGACTACTTCTCTCTCGTCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K110985-0022-092-A07C-09ew
TCTCTCTAGTTTCAGATAATTTCTATTTCAGAGATGATGATGATTAGGGAATGTGAAGCC
CAAGCCCAATTTCTAAACACCGTGTCGTTTTGATAGCTAAATAACAGTGAGATGCTGAGT
CGTGAAACGGCACGCAGTGTTTTGTTTTTTTAAACATTGAATTTCTCGGCCCGACATTAT
CGTGGAAGTACGAGATTCACTGGATTTGATGGCTCGTCGGCTGTTTCTCCTCCTCCTCGC
CGTCACGGCAGGGTTGAGCTTGACGGGAACAACCGTCAGAGCAGTTGGCATAAATTGGGG
GACAGAAGCGTCGCACCCTCTTCCGCCTTCAAAGGTTGTAGAGCTTCTGAAATCAAACGG
CATTGTCAAAGTGAAGCTCTTCGATGCCGACCCAAAAGTTCTCCGAGCTCTCTCTGGTTC
TAATATTGGTGTCACTATCGGAATCCAAAATTCGATGCTCAAGAGCTTGAATGCATCCGT
AAAGGTAGCAGAGAGCTGGGTACATGACAATGTCACTCGTTACTTCAACGGTGGAAACAG
AGTTCGAATCGAGTATCTTCTTTCTCCTTCTCTTCTGCTTATCCAGTTATATATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTATATATCC
GCAC
   
Sequence Name 11-K110984-0022-092-A07C-8409
GenBank Accession LN491171 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K110984-0022-092-A07C-8409
TTACAATTGAATATATATATAACTGGATAAGCAGAAGAGAAGGAGAAAGAAGATACTCGA
TTCGAACTCTGTTTCCACCGTTGAAGTAACGAGTGACATTGTCATGTACCCAGCTCTCTG
CTACCTTTACGGATGCATTCAAGCTCTTGAGCATCGAATTTTGGATTCCGATAGTGACAC
CAATATTAGAACCAGAGAGAGCTCGGAGAACTTTTGGGTCGGCATCGAAGAGCTTCACTT
TGACAATGCCGTTTGATTTCAGAAGCTCTACAACCTTTGAAGGCGGAAGAGGGTGCGACG
CTTCTGTCCCCCAATTTATGCCAACTGCTCTGACGGTTGTTCCCGTCAAGCTCAACCCTG
CCGTGACGGCGAGGAGGAGGAGAAACAGCCGACGAGCCATCAAATCCAGTGAATCTCGTA
CTTCCACGATAATGTCGGGCCGAGAAATTCAATGTTTAAAAAAACAAAACACTGCGTGCC
GTTTCACGACTCAGCATCTCACTGTTATTTAGCTATCAAAACGACACGGTGTTTAGAAAT
TGGGCTTGGGCTTCACATTCCCTAATCATCATCATCTCTGAAATAGAAATTATCTGAAAC
TTAGAGAGACAGAGAGAGAGAAAGCTCAAATTCAATCATCAATGTTAACGGACGAGAGAA
AAGTAGTCTG
Primers used for PCR 05ez - 8474, show primer details
   
Sequence Name 31-K111117-0022-092-A07C-05ez
GenBank Accession LN500214 [GenBank]
Sequencing primer gene specific primer, 05ez: 5'-ACAGACTACTTCTCTCTCGTCCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K111117-0022-092-A07C-05ez
CTGTCTCTCTAGTTTCAGATAATTTCTATTTCAGAGATGATGATGATTAGGGAATGTGAA
GCCCAAGCCCAATTTCTAAACACCGTGTCGTTTTGATAGCTAAATAACAGTGAGATGCTG
AGTCGTGAAACGGCACGCAGTGTTTTGTTTTTTTAAACATTGAATTTCTCGGCCCGACAT
TATCGTGGAAGTACGAGATTCACTGGATTTGATGGCTCGTCGGCTGTTTCTCCTCCTCCT
CGCCGTCACGGCAGGGTTGAGCTTGACGGGAACAACCGTCAGAGCAGTTGGCATAAATTG
GGGGACAGAAGCGTCGCACCCTCTTCCGCCTTCAAAGGTTGTAGAGCTTCTGAAATCAAA
CGGCATTGTCAAAGTGAAGCTCTTCGATGCCGACCCAAAAGTTCTCCGAGCTCTCTCTGG
TTCTAATATTGGTGTCACTATCGGAATCCAAAATTCGATGCTCAAGAGCTTGAATGCATC
CGTAAAGGTAGCAGAGAGCTGGGTACATGACAATGTCACTCGTTACTTCAACGGTGGAAA
CAGAGTTCGAATCGAGTATCTTCTTTCTCCTTCTCTTCTGCTTATCCAGTTATATATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCC
   
Sequence Name 15-K111116-0022-092-A07C-8409
GenBank Accession LN493027 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K111116-0022-092-A07C-8409
TATATAACTGGATAAGCAGAAGAGAAGGAGAAAGAAGATACTCGATTCGAACTCTGTTTC
CACCGTTGAAGTAACGAGTGACATTGTCATGTACCCAGCTCTCTGCTACCTTTACGGATG
CATTCAAGCTCTTGAGCATCGAATTTTGGATTCCGATAGTGACACCAATATTAGAACCAG
AGAGAGCTCGGAGAACTTTTGGGTCGGCATCGAAGAGCTTCACTTTGACAATGCCGTTTG
ATTTCAGAAGCTCTACAACCTTTGAAGGCGGAAGAGGGTGCGACGCTTCTGTCCCCCAAT
TTATGCCAACTGCTCTGACGGTTGTTCCCGTCAAGCTCAACCCTGCCGTGACGGCGAGGA
GGAGGAGAAACAGCCGACGAGCCATCAAATCCAGTGAATCTCGTACTTCCACGATAATGT
CGGGCCGAGAAATTCAATGTTTAAAAAAACAAAACACTGCGTGCCGTTTCACGACTCAGC
ATCTCACTGTTATTTAGCTATCAAAACGACACGGTGTTTAGAAATTGGGCTTGGGCTTCA
CATTCCCTAATCATCATCATCTCTGAAATAGAAATTATCTGAAACTTAGAGAGACAGAGA
GAGAGAAAGCTCAAATTCAATCATCAATGTTAACGGACGAGAGAGAA

Last Updated on 06.12.2018 16:11